Explore
Validate
Learn
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunocytochemistry [2]
- Immunohistochemistry [2]
Submit
Validation data
Reference
Comment
Report error
- Product number
- GTX109389 - Provider product page
- Provider
- GeneTex
- Proper citation
- GeneTex Cat#GTX109389, RRID:AB_1950010
- Product name
- Caspr2 antibody [N2N3]
- Antibody type
- Polyclonal
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
No comments: Submit comment
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Sample (50 ?g of whole cell lysate) A: Mouse brain 5% SDS PAGE GTX109389 diluted at 1:10000 The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Sample (30 ?g of whole cell lysate)A: MOLT4 (GTX27912)5% SDS PAGEGTX109389 diluted at 1:5000The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Caspr2 antibody [N2N3] detects Caspr2 protein at cytoplasm by immunofluorescent analysis.Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: Caspr2 protein stained by Caspr2 antibody [N2N3] (GTX109389) diluted at 1:200.Blue: Hoechst 33342 staining.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Caspr2 antibody [N2N3] detects Caspr2 protein by immunofluorescent analysis.Sample: DIV14 rat E18 primary cortical neurons were fixed in 4% paraformaldehyde at RT for 15 min.Green: Caspr2 protein stained by Caspr2 antibody [N2N3] (GTX109389) diluted at 1:500.Red: beta Tubulin 3/ Tuj1, stained by beta Tubulin 3/ Tuj1 antibody [GT1338] (GTX631831) diluted at 1:500.Blue: Fluoroshield with DAPI (GTX30920).
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Caspr2 antibody [N2N3] detects Caspr2n proteins in embryonic mouse brain by immunohistochemical analysis. Sample: Frozen section of embryonic mouse brain (mE18.5). Red: Caspr2 antibody [N2N3] (GTX109389) diluted at 1:250. Blue: DAPI.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Caspr2 antibody [N2N3] detects Caspr2 protein expression by immunohistochemical analysis.Sample: Frozen sectioned E13.5 Rat brain. Green: Caspr2 protein stained by Caspr2 antibody [N2N3] (GTX109389) diluted at 1:250.Red: beta Tubulin 3/ TUJ1, a mature neuron marker, stained by beta Tubulin 3/ TUJ1 antibody [GT11710] (GTX631836) diluted at 1:500.Blue: Fluoroshield with DAPI (GTX30920).
