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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunohistochemistry [1]
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- Product number
- AF5145 - Provider product page
- Provider
- R&D Systems
- Product name
- Human/Mouse/Rat Caspr2 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. Detects human Caspr2 in direct ELISAs and human, mouse, and rat Caspr2 in Western blots. In direct ELISAs and Western blots, less than 1% cross-reactivity with recombinant human Caspr1 is observed.
- Reactivity
- Human, Mouse, Rat
- Host
- Sheep
- Conjugate
- Unconjugated
- Antigen sequence
Q9UHC6- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of Human, Mouse, and Rat Caspr2 by Western Blot. Western blot shows lysates of human brain tissue, mouse brain tissue, and rat brain tissue. PVDF membrane was probed with 2 µg/mL of Sheep Anti-Human/Mouse/Rat Caspr2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5145) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Caspr2 at approximately 160-170 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Caspr2 in Mouse Brain. Caspr2 was detected in perfusion fixed frozen sections of mouse brain using 10 µg/mL Sheep Anti-Human/Mouse/Rat Caspr2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5145) overnight at 4 °C. Tissue was stained with the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red; Catalog # NL010) and counterstained with DAPI (blue). View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections.
