Antibody data
- Antibody Data
- Antigen structure
- References [3]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
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Validation data
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- Product number
- PAB10251 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#PAB10251, RRID:AB_1676575
- Product name
- IRAK4 polyclonal antibody
- Antibody type
- Polyclonal
- Antigen
- A synthetic peptide corresponding to C-terminus of human IRAK4.
- Reactivity
- Human
- Host
- Rabbit
- Vial size
- 100 μg
- Storage
- Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.
Submitted references IRAK-M is a novel member of the Pelle/interleukin-1 receptor-associated kinase (IRAK) family.
IRAK (Pelle) family member IRAK-2 and MyD88 as proximal mediators of IL-1 signaling.
IRAK: a kinase associated with the interleukin-1 receptor.
Wesche H, Gao X, Li X, Kirschning CJ, Stark GR, Cao Z
The Journal of biological chemistry 1999 Jul 2;274(27):19403-10
The Journal of biological chemistry 1999 Jul 2;274(27):19403-10
IRAK (Pelle) family member IRAK-2 and MyD88 as proximal mediators of IL-1 signaling.
Muzio M, Ni J, Feng P, Dixit VM
Science (New York, N.Y.) 1997 Nov 28;278(5343):1612-5
Science (New York, N.Y.) 1997 Nov 28;278(5343):1612-5
IRAK: a kinase associated with the interleukin-1 receptor.
Cao Z, Henzel WJ, Gao X
Science (New York, N.Y.) 1996 Feb 23;271(5252):1128-31
Science (New York, N.Y.) 1996 Feb 23;271(5252):1128-31
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Western blot using IRAK4 polyclonal antibody (Cat # PAB10251) shows detection of IRAK4 in HeLa whole cell lysate. The membrane was probed with the primary antibody dilutedto 1:1000 (lane 1), 1 : 500 (lane 2), and 1 : 250 (lane 3) respectively.
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Immunocytochemistry using IRAK4 polyclonal antibody (Cat # PAB10251) shows staining of IRAK4 in HeLa cells.The cells were fixed with paraformaldehyde and reacted with the primary antibody diluted to 1 : 100.
- Validation comment
- Immunocytochemistry