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Western blot
ELISAAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunocytochemistry [1]
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- Product number
- AP09337PU-N - Provider product page
- Provider
- Acris Antibodies GmbH
- Proper citation
- Acris Antibodies GmbH Cat#AP09337PU-N, RRID:AB_2035699
- Product name
- anti IRAK4
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide corresponding to a region near the carboxy terminus of human IRAK-4 protein
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.1 mg
- Concentration
- 1.0 mg/ml (by UV absorbance at 280 nm)
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Supportive validation
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Western blot using affinity purified anti-IRAK-4 antibody shows detection of IRAK-4 in HeLa whole cell lysate. The membrane was probed with the primary antibody diluted to 1:1000 (lane 1), 1:500 (lane 2), and 1:250 (lane 3) respectively.
Supportive validation
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Immunohistochemistry using Affinity Purified anti-IRAK-4 antibody shows staining of IRAK-4 in HeLa cells. The cells were fixed with paraformaldehyde and reacted with the primary antibody diluted to 1:100.
