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- Antibody Data
- Antigen structure
- References [4]
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- Validations
- Flow cytometry [1]
- Blocking/Neutralizing [1]
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- Product number
- AF773 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Goat Polyclonal IFN-gamma R2 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. Detects human IFN-gamma R2 in direct ELISAs and Western blots. In direct ELISAs and Western blots, less than 1% cross-reactivity with recombinant human IFN-gamma R1 is observed.
- Reactivity
- Human
- Host
- Goat
- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.
Submitted references Aberrant expression of NKL homeobox gene HLX in Hodgkin lymphoma.
Trisomy 21 consistently activates the interferon response.
Glycosylation-Dependent IFN-γR Partitioning in Lipid and Actin Nanodomains Is Critical for JAK Activation.
Immunologic analysis of HIV-uninfected Taiwanese children with BCG-induced disease.
Nagel S, Pommerenke C, Meyer C, Kaufmann M, MacLeod RAF, Drexler HG
Oncotarget 2018 Mar 6;9(18):14338-14353
Oncotarget 2018 Mar 6;9(18):14338-14353
Trisomy 21 consistently activates the interferon response.
Sullivan KD, Lewis HC, Hill AA, Pandey A, Jackson LP, Cabral JM, Smith KP, Liggett LA, Gomez EB, Galbraith MD, DeGregori J, Espinosa JM
eLife 2016 Jul 29;5
eLife 2016 Jul 29;5
Glycosylation-Dependent IFN-γR Partitioning in Lipid and Actin Nanodomains Is Critical for JAK Activation.
Blouin CM, Hamon Y, Gonnord P, Boularan C, Kagan J, Viaris de Lesegno C, Ruez R, Mailfert S, Bertaux N, Loew D, Wunder C, Johannes L, Vogt G, Contreras FX, Marguet D, Casanova JL, Galès C, He HT, Lamaze C
Cell 2016 Aug 11;166(4):920-934
Cell 2016 Aug 11;166(4):920-934
Immunologic analysis of HIV-uninfected Taiwanese children with BCG-induced disease.
Lee WI, Liang FC, Huang JL, Jaing TH, Wang CH, Lin TY, Huang YC, Huang WL, Jou R, Hsieh MY, Chia JH, Wu TL
Journal of clinical immunology 2009 May;29(3):319-29
Journal of clinical immunology 2009 May;29(3):319-29
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Detection of IFN-gamma R2 in human blood-derived granulocytes Blood-derived granulocytes were stained with Goat Anti-Human IFN-gamma R2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF773, filled histogram) or control antibody (Catalog # AB-108-C, open histogram), followed by Allophycocyanin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0108).
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- IFN-gamma Inhibition of EMCV-induced Cytopathy and Neutralization by Human IFN-gamma R2 Antibody. Recombinant Human IFN-gamma (Catalog # 285-IF) reduces the Encephalomyocarditis Virus (EMCV)-induced cytopathy in the HeLa human cervical epithelial carcinoma cell line in a dose-dependent manner (orange line). Inhibition of EMCV activity elicited by Recombinant Human IFN-gamma (2 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human IFN-gamma R2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF773). The ND50 is typically 0.5-2.5 µg/mL.
