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Western blot
ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
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- Product number
- PA5-34959 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- ENY2 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: NT2D1, PC-3, U87-MG, SK-N-SH. Predicted reactivity: Mouse (100%), Rat (100%), Zebrafish (86%), Xenopus laevis (93%), Pig (100%), Chicken (96%), Rhesus Monkey (100%), Bovine (100%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 0.62 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of ENY2 using A) 30 µg NT2D1 whole cell lysate (B) 30 µg PC-3 whole cell lysate (C) 30 µg U87-MG whole cell lysate and D) 30 µg SK-N-SH whole cell lysate. Samples were loaded onto a 15% SDS-PAGE gel and probed with an ENY2 polyclonal antibody (Product # PA5-34959) at a dilution of 1:1000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using ENY2 Polyclonal Antibody (Product # PA5-34959). Various whole cell extracts (30 µg) were separated by 15% SDS-PAGE, and the membrane was blotted with ENY2 Polyclonal Antibody (Product # PA5-34959) diluted at 1:1,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
