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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
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- Product number
- PA5-95817 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- RNF152 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- Immunogen sequence: YMLPLPISKE RALLPGDMGC RLLPGSQQKS VTVVTIPAEQ QPLQGGAPQE AVEEEQDRRG VVKSSTWSGV CTVILVACVL VFLLGIVLHN MSCISKRFTV I; Positive Samples: U-251MG, 293T, LO2; Cellular Location: Lysosome membrane, Single-pass membrane protein
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 2.26 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
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Supportive validation
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- Invitrogen Antibodies (provider)
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- Experimental details
- Western Blot analysis of RNF152 in extracts of various cell lines using RNF152 Polyclonal Antibody (Product # PA5-95817) at a dilution of 1:1000. A HRP Goat Anti-Rabbit IgG (H+L) secondary antibody was used at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST.
