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Western blot
ELISAAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [2]
- Immunoprecipitation [1]
- Immunohistochemistry [1]
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- Product number
- 11927-1-AP - Provider product page
- Provider
- Proteintech Group
- Proper citation
- Proteintech Cat#11927-1-AP, RRID:AB_2098440
- Product name
- ERGIC2 antibody
- Antibody type
- Polyclonal
- Description
- ERGIC2 antibody (Cat. #11927-1-AP) is a rabbit polyclonal antibody that shows reactivity with human and has been validated for the following applications: IF, IHC, IP, WB, ELISA.
- Reactivity
- Human
- Host
- Rabbit
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 20ul, 150ul
Submitted references Characterization of a variant of ERGIC2 transcript.
Kwok SC, Kumar S, Dai G
DNA and cell biology 2014 Feb;33(2):73-8
DNA and cell biology 2014 Feb;33(2):73-8
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Supportive validation
Supportive validation
- Submitted by
- kwokscm
- Main image
- Experimental details
- The ERGIC2 antibody was tested with protein extract from three human cancer cell lines, PC-3, MCF7 and DU145, and one rat cancer cell line, PC-12. In one sample, the PC-3 cells were treated with 100 ng/ml brefeldin A for 2 days. Brefeldin A is known to inhibit protein transport in ER, and this may result in accumulation of ERGIC2 in ER.
- Sample type
- PC-3, MCF-7, and DU145 human cancer cell lines; PC-12 rat cancer cell line
- Primary Ab dilution
- 1:1000
- Secondary Ab
- Secondary Ab
- Secondary Ab dilution
- 1:2000
Supportive validation
- Submitted by
- Proteintech Group (provider)
- Main image
- Experimental details
- HepG2 cells were subjected to SDS PAGE followed by western blot with 11927-1-AP(ERGIC2 antibody) at dilution of 1:500
- Sample type
- cell line
Supportive validation
- Submitted by
- Proteintech Group (provider)
- Main image
- Experimental details
- IP Result of anti-ERGIC2 (IP:11927-1-AP, 4ug; Detection:11927-1-AP 1:1000) with HepG2 cells lysate 2900ug.
- Sample type
- cell line
Supportive validation
- Submitted by
- Proteintech Group (provider)
- Main image
- Experimental details
- The ERGIC2 antibody from Proteintech is a rabbit polyclonal antibody to a recombinant protein of human ERGIC2. This antibody recognizes human,mouse,rat antigen. The ERGIC2 antibody has been validated for the following applications: ELISA, WB, IHC, IP analysis.
