AF5687

antibody from R&D Systems

Targeting: STX5 SED5, STX5A

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

AF5687

Provider

R&D Systems

Product name

Human/Rat Syntaxin 5 Antibody

Antibody type

Polyclonal

Description

Antigen Affinity-purified. Detects human Syntaxin 5 in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross-reactivity with recombinant human (rh) Syntaxin 7, rhSyntaxin 8, and rhSytaxin 12 is observed.

Reactivity

Human, Rat

Host

Goat

Conjugate

Unconjugated

Antigen sequence

Q13190


Isotype

IgG

Vial size

100 ug

Concentration

LYOPH

Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Western blot

Supportive validation

Submitted by

R&D Systems (provider)

Main image

Experimental details

Detection of Human Syntaxin 5 by Western Blot. Western blot shows lysates of T47D human breast cancer cell line and Raji human Burkitt's lymphoma cell line. PVDF membrane was probed with 0.5 µg/mL of Goat Anti-Human/Rat Syntaxin 5 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5687) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). Specific bands were detected for Syntaxin 5 at approximately 35 and 42 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Supportive validation

Submitted by

R&D Systems (provider)

Main image

Experimental details

Detection of Human Syntaxin 5 by Simple Western^TM. Simple Western lane view shows lysates of T47D human breast cancer cell line and Raji human Burkitt's lymphoma cell line, loaded at 0.2 mg/mL. Specific bands were detected for Syntaxin 5 at approximately 39 and 47 kDa (as indicated) using 25 µg/mL of Goat Anti-Human/Rat Syntaxin 5 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5687) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Immunocytochemistry

Supportive validation

Submitted by

R&D Systems (provider)

Main image

Experimental details

Syntaxin 5 in SH-SY5Y Human Cell Line. Syntaxin 5 was detected in immersion fixed SH-SY5Y human neuroblastoma cell line using Goat Anti-Human/Rat Syntaxin 5 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5687) at 1.7 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Immunohistochemistry

Supportive validation

Submitted by

R&D Systems (provider)

Main image

Experimental details

Syntaxin 5 in Human Brain. Syntaxin 5 was detected in immersion fixed paraffin-embedded sections of human brain (substantia nigra) using Goat Anti-Human/Rat Syntaxin 5 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5687) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in neurons. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Supportive validation

Submitted by

R&D Systems (provider)

Main image

Experimental details

Syntaxin 5 in Rat Brain. Syntaxin 5 was detected in immersion fixed frozen sections of rat brain using Goat Anti-Human/Rat Syntaxin 5 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5687) at 3 µg/mL overnight at 4 °C. Tissue was stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to neuronal cytoplasm. View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections.