Antibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
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Validation data
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- Product number
- AP32473SU-N - Provider product page
- Provider
- Acris Antibodies GmbH
- Product name
- anti FAK1 / PTK2 pSer789
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide corresponding to amino acids 571-582 of Human FAK (MEDST[pY] YKASKG-C).
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.2 ml
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Supportive validation
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- Acris Antibodies GmbH (provider)
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- Experimental details
- Western Blot Analysis: Lanes 1 and 2 demonstarate untransformed and Src (527F)-transformed Rat2 cells(lanes 1 and 2, respectively) Lanes 3-6 demonstrate untreated (lanes 3 and 5) and pervanadate-treated (lanes 4 and 6) C2C12 (lanes 3 and 4) and HUVEC (lanes 5 and 6) cell lysate resolved by SDS-PAGE, transferred to PVDF, and probed with anti phospho-FAK (Tyr576) (1:500) Proteins were visualized using a donkey anti rabbit secondary antibody conjugated to HRP and a chemiluminescence detection system. Arrow indicates FAK (~125kDa).