Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
- Flow cytometry [1]
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Validation data
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- Product number
- PA5-35348 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- ESRRA Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- This antibody is predicted to react with mouse and rat based on sequence homology.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 400 µL
- Concentration
- 0.45 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of ESRRA in 293 cell lysate (2 µg/lane) either nontransfected (lane 1) or transiently transfected (lane 2) with the ESRRA gene using an ESRRA polyclonal antibody (Product # PA5-35348).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of ESRRA in A375 cell lysate (35 µg/lane) using an ESRRA polyclonal antibody (Product # PA5-35348).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of ESRRA in 293 cells using an ESRRA polyclonal antibody (Product # PA5-35348) followed by detection using a fluorescent conjugated secondary antibody (green). Nuclei were stained with Dapi (blue).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of ESRRA in formalin-fixed, paraffin-embedded human kidney carcinoma using an ESRRA polyclonal antibody (Product # PA5-35348) followed by DAB staining.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow cytometry analysis of ESRRA in 293 cells (bottom) compared to a negative control (top) using an ESRRA polyclonal antibody (Product # PA5-35348) followed by detection using a FITC-conjugated goat-anti-rabbit secondary antibody.