- AMAb90733 - Atlas Antibodies HNF1B antibody

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Enhanced validation

Submitted by: Atlas Antibodies (provider)
Enhanced method: Orthogonal validation
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Experimental details: Western blot analysis in human cell lines Caco-2 and A-431 using Anti-HNF1B antibody. Corresponding HNF1B RNA-seq data are presented for the same cell lines. Loading control: Anti-PARP1.
Orthogonal method: Orthogonal validation of protein expression using WB by comparison to RNA-seq data of corresponding target in high and low expression cell lines.

Supportive validation

Submitted by: Atlas Antibodies (provider)
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Experimental details: Lane 1: Marker [kDa]Lane 2: Negative control (vector only transfected HEK293T lysate)Lane 3: HNF1B Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY400162)

Supportive validation

Submitted by: Atlas Antibodies (provider)
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Experimental details: Immunofluorescence staining in A549 cell line with Anti-HNF1B monoclonal antibody, showing spotty nuclear (without nucleoli) staining in green. Microtubule probes are visualized in red (where available).
Sample type: HUMAN

Enhanced validation

Submitted by: Atlas Antibodies (provider)
Enhanced method: Orthogonal validation
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Experimental details: Immunohistochemistry analysis in human kidney and tonsil tissues using AMAb90733 antibody. Corresponding HNF1B RNA-seq data are presented for the same tissues.
Sample type: HUMAN
Orthogonal method: Orthogonal validation of protein expression using IHC by comparison to RNA-seq data of corresponding target in high and low expression tissues.

Supportive validation

Submitted by: Atlas Antibodies (provider)
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Experimental details: Immunohistochemical staining of human kidney shows moderate to strong nuclear immunoreactivity in renal tubules.

Supportive validation

Submitted by: Atlas Antibodies (provider)
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Experimental details: Immunohistochemical staining of human pancreas shows nuclear positivity in pancreatic ducts.

Supportive validation

Submitted by: Atlas Antibodies (provider)
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Experimental details: Immunohistochemical staining of human small intestine shows nuclear immunoreactivity in glandular cells.

Supportive validation

Submitted by: Atlas Antibodies (provider)
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Experimental details: Immunohistochemical staining of human liver shows strong nuclear positivity in bile ducts.

Supportive validation

Submitted by: Atlas Antibodies (provider)
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Experimental details: Immunohistochemical staining of human uterus shows strong nuclear immunoreactivity in glandular cells.

Supportive validation

Submitted by: Atlas Antibodies (provider)
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Experimental details: Immunohistochemical staining of human tonsil shows absence of staining in both lymphoid and squamose epithelial cells (negative control).

Supportive validation

Submitted by: Atlas Antibodies (provider)
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Experimental details: Immunohistochemical staining of human kidney shows strong nuclear positivity in cells in tubules.

Supportive validation

Submitted by: Atlas Antibodies (provider)
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Experimental details: Immunohistochemical staining of human colon shows moderate to strong nuclear positivity in glandular cells.

Supportive validation

Submitted by: Atlas Antibodies (provider)
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Experimental details: Immunohistochemical staining of human renal cancer shows strong nuclear positivity in tumor cells.

Supportive validation

Submitted by: Atlas Antibodies (provider)
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Experimental details: Immunohistochemical staining of human colorectal cancer shows moderate nuclear positivity in tumor cells.

Supportive validation

Submitted by: Atlas Antibodies (provider)
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Experimental details: Immunohistochemical staining of human tonsil shows no positivity in lymphoid cells as expected.

AMAb90733