Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [3]
- Immunohistochemistry [3]
- Other assay [1]
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Validation data
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- Product number
- A301-225A - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- CHD8 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Other
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 0.20 mg/mL
- Storage
- 4° C
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Detection of human and mouse CHD8 by western blot. Samples: Whole cell lysate (50 µg) from HEK293T, K-562, MCF-7, and NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified Rabbit anti-CHD8 antibody (Product # A301-225A; lot A301-225A-4) used for WB at 0.1 µg/mL. Detection: Chemiluminescence with an exposure time of 75 seconds.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Detection of human CHD8 by western blot of immunoprecipitates. Samples: Whole cell lysate (50 µg) prepared using NETN buffer from 293T cells. Antibodies: Affinity purified rabbit anti-CHD8 antibody A301-225A (lot A301-225A-3) used for IP at 6 µg per reaction. CHD8 was also immunoprecipitated by a previous lot of this antibody (lot A301-225A-2) and rabbit anti-CHD8 antibody A301-224A. For blotting immunoprecipitated CHD8, A301-225A-3 was used at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Detection of human and mouse CHD8 by western blot. Samples: Whole cell lysate (50 µg) prepared using NETN buffer from HeLa, 293T, Jurkat, mouse TCMK-1, and mouse NIH3T3 cells. Antibodies: Affinity purified rabbit anti-CHD8 antibody A301-225A (lot A301-225A-3) used for WB at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Detection of human CHD8 by immunohistochemistry. Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti-CHD8 (Product # A301-225A lot 4) used at a dilution of 1:1000 (0.2 µg/mL). Detection: DAB.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Detection of mouse CHD8 by immunohistochemistry. Sample: FFPE section of mouse CT26 colon carcinoma. Antibody: Affinity purified rabbit anti-CHD8 (Product # A301-225A; lot 4) used at a dilution of 1:1000 (0.2 µg/mL). Detection: DAB.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Detection of human CHD8 by immunohistochemistry. Sample: FFPE section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti-CHD8 (Cat. No. A301-225A Lot3) used at a dilution of 1:200 (1µg/ml). Detection: DAB.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Detection of human CHD8 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HEK293T cells prepared using NETN lysis buffer. Antibodies: Rabbit anti-CHD8 antibody (Product # A301-225A; lot A301-225A-4) used for IP at 6 µL per reaction. CHD8 was also immunoprecipitated by a previous lot of this antibody (lot A301-225A-3). For blotting immunoprecipitated CHD8 (Product # A301-225A) was used at 0.1 µg/mL. Chemiluminescence with an exposure time of 1 second.