PA5-35321

antibody from Invitrogen Antibodies

Targeting: GTF2I BAP-135, BTKAP1, DIWS, IB291, SPIN, TFII-I, WBSCR6

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-35321

Provider

Invitrogen Antibodies

Product name

GTF2I Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Synthetic peptide

Description

This antibody is predicted to react with rat based on sequence homology.

Reactivity

Human

Host

Rabbit

Isotype

IgG

Vial size

400 µL

Storage

-20° C, Avoid Freeze/Thaw Cycles

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of GTF2I in Hela cell lysate (35 µg/lane) using a GTF2I polyclonal antibody (Product # PA5-35321).

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of GTF2I showing staining in the nucleus of A549 cells using a GTF2I polyclonal antibody (Product # PA5-35321) followed by detection using a fluorescent conjugated secondary antibody (green). Cytoplasmic actin was stained with a fluorescent red phalloidin (7units/mL, 1 h at 37ºC).

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of GTF2I in formalin-fixed, paraffin-embedded human brain tissue using a GTF2I polyclonal antibody (Product # PA5-35321) followed by DAB staining.

Flow cytometry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Flow cytometry analysis of GTF2I in K562 cells (bottom) compared to a negative control (top) using a GTF2I polyclonal antibody (Product # PA5-35321) followed by detection using a FITC-conjugated goat-anti-rabbit secondary antibody.