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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- Immunohistochemistry [1]
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- Product number
- MAB9535-100 - Provider product page
- Provider
- R&D Systems
- Product name
- Human DUX4 Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from cell culture supernatant. Detects human DUX4 in direct ELISAs and Western blots.
- Reactivity
- Human
- Host
- Rabbit
- Conjugate
- Unconjugated
- Isotype
- IgG
- Antibody clone number
- 2142A
- Vial size
- 100 ug
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of Human DUX4 by Western Blot. Western blot shows lysates of C2C12 mouse myoblast cell line transfected with human DUX4 untreated (-) or treated (+) with Doxycycline. PVDF membrane was probed with 0.1 µg/mL of Rabbit Anti-Human DUX4 Monoclonal Antibody (Catalog # MAB9535) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for DUX4 at approximately 55 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of Human DUX4 by Simple WesternTM. Simple Western lane view shows lysates of HEK293 human embryonic kidney cell line either mock transfected or transfected with human DUX4 and untreated (-) or treated (+) with Doxycycline, loaded at 0.2 mg/mL. A specific band was detected for DUX4 at approximately 62 kDa (as indicated) using 20 µg/mL of Rabbit Anti-Human DUX4 Monoclonal Antibody (Catalog # MAB9535). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- DUX4 in Human Testis. DUX4 was detected in immersion fixed paraffin-embedded sections of human testis using Rabbit Anti-Human DUX4 Monoclonal Antibody (Catalog # MAB9535) at 3 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Rabbit IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC003). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to nuclei. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.
