Explore
Validate
Learn
Western blot
ELISAAntibody data
- Antibody Data
- Antigen structure
- References [4]
- Comments [0]
- Validations
- Western blot [3]
- Immunohistochemistry [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- NBP1-49552 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NBP1-49552, RRID:AB_11019332
- Product name
- Mouse Monoclonal DUX4 Antibody
- Antibody type
- Monoclonal
- Description
- Protein G purified. This antibody is specific for DUX4 and does not recognize DUX4c.
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 0.1 ml
- Concentration
- 1 mg/ml
- Storage
- Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Submitted references Transcriptomic pathway analysis of urokinase receptor silenced breast cancer cells: a microarray study.
Concordant but Varied Phenotypes among Duchenne Muscular Dystrophy Patient-Specific Myoblasts Derived using a Human iPSC-Based Model.
Immunodetection of human double homeobox 4.
Facioscapulohumeral dystrophy: incomplete suppression of a retrotransposed gene.
Narayanaswamy PB, Baral TK, Haller H, Dumler I, Acharya K, Kiyan Y
Oncotarget 2017 Nov 24;8(60):101572-101590
Oncotarget 2017 Nov 24;8(60):101572-101590
Concordant but Varied Phenotypes among Duchenne Muscular Dystrophy Patient-Specific Myoblasts Derived using a Human iPSC-Based Model.
Choi IY, Lim H, Estrellas K, Mula J, Cohen TV, Zhang Y, Donnelly CJ, Richard JP, Kim YJ, Kim H, Kazuki Y, Oshimura M, Li HL, Hotta A, Rothstein J, Maragakis N, Wagner KR, Lee G
Cell reports 2016 Jun 7;15(10):2301-2312
Cell reports 2016 Jun 7;15(10):2301-2312
Immunodetection of human double homeobox 4.
Geng LN, Tyler AE, Tapscott SJ
Hybridoma (2005) 2011 Apr;30(2):125-30
Hybridoma (2005) 2011 Apr;30(2):125-30
Facioscapulohumeral dystrophy: incomplete suppression of a retrotransposed gene.
Snider L, Geng LN, Lemmers RJ, Kyba M, Ware CB, Nelson AM, Tawil R, Filippova GN, van der Maarel SM, Tapscott SJ, Miller DG
PLoS genetics 2010 Oct 28;6(10):e1001181
PLoS genetics 2010 Oct 28;6(10):e1001181
No comments: Submit comment
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Simple Western: DUX4 Antibody (P4H2) [NBP1-49552] - Lane view shows a specific band for DUX4 in 0.5 mg/ml of HeLa lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: DUX4 Antibody (P4H2) [NBP1-49552] - Analysis of DUX4 expression in (1) human testis and (2) DUX4 protein lysates.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: DUX4 Antibody (P4H2) [NBP1-49552] - Total protein from human testis tissue and human K562, HeLa and HepG2 cell lines was separated on a 12% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/ml anti-DUX4 in 1% non-fat milk in TBST and detected with an anti-mouse HRP secondary antibody using chemiluminescence.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry: DUX4 Antibody (P4H2) [NBP1-49552] - Analysis of DUX4 on human testis
