Antibody data
- Antibody Data
- Antigen structure
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- Validations
- Other assay [1]
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Validation data
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- Product number
- 687605 - Provider product page
- Provider
- BioLegend
- Proper citation
- BioLegend Cat#687605, RRID:AB_2687115
- Product name
- Go-ChIP-Grade™ Purified anti-Histone H3.1 Phospho (Ser28)
- Antibody type
- Monoclonal
- Reactivity
- Human
- Host
- Rat
- Conjugate
- Unconjugated
- Antibody clone number
- 5D10D4
- Vial size
- 25 µg
- Concentration
- 0.5 mg/ml
- Storage
- 2°C-8°C
- Handling
- Ambient RT
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Supportive validation
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- BioLegend (provider)
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- Experimental details
- Chromatin Immunoprecipitation (ChIP) was performed using commercial Protein-G coated 96 well high-throughput ChIP assay kit by loading 3µg of cross-linked chromatin samples from HeLa cells treated with Nocodazole with either A) 1:50 dilution of Go-ChIP-Grade™ Purified anti-Histone H3.1 Phospho (Ser28) Antibody (Clone 5D10D4), B) equal amount of Purified Rat IgG2a, κ Isotype Control Antibody, or C) competitor’s ChIP-grade Purified anti-Histone H3.1 Phospho (Ser28) Antibody and D) equal amount of matched Isotype Control Antibody as recommended by the manufacturer. The enriched DNA was purified and quantified by real-time qPCR using primers targeting human TNF&alpha gene region. The amount of immunoprecipitated DNA in each sample is represented as signal relative to the 5% of total amount of input chromatin.
- Conjugate
- Unconjugated