Antibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [4]
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- Product number
- MA5-18074 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- H4K20me3 Monoclonal Antibody (6F8-D9)
- Antibody type
- Monoclonal
- Antigen
- Synthetic peptide
- Description
- This antibody shows weak cross-reactivity with K20 di-methylated histone H4, but does not react with histone H3 or mono- or unmethylated K20 histone 4. This antibody is predicted to react with Xenopus laevis, Arabidopsis thaliana, Fruit fly (Drosophila melanogaster), and mammals. This target has a predicted molecular weight of 11 kDa.
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- 6F8-D9
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of Tri-Methyl Histone H4 (Lys20) in Hela cell lysates using a Tri-Methyl Histone H4 (Lys20) monoclonal antibody (Product # MA5-18074) at various dilutions, followed by detection using an AP-conjugated mouse IgG whole molecule antibody at a dilution of 1:2000 on a BCIP/NBT substrate.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of Tri-Methyl Histone H4 (Lys20) in Hela cell lysates using a Tri-Methyl Histone H4 (Lys20) monoclonal antibody (Product # MA5-18074) at various dilutions, followed by detection using an AP-conjugated mouse IgG whole molecule antibody at a dilution of 1:2000 on a BCIP/NBT substrate.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of Tri-Methyl Histone H4 (Lys20) in Hela cell lysates using a Tri-Methyl Histone H4 (Lys20) monoclonal antibody (Product # MA5-18074) at various dilutions, followed by detection using an AP-conjugated mouse IgG whole molecule antibody at a dilution of 1:2000 on a BCIP/NBT substrate.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of Tri-Methyl Histone H4 (Lys20) in Hela cell lysates using a Tri-Methyl Histone H4 (Lys20) monoclonal antibody (Product # MA5-18074) at various dilutions, followed by detection using an AP-conjugated mouse IgG whole molecule antibody at a dilution of 1:2000 on a BCIP/NBT substrate.