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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [3]
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- Validations
- Western blot [2]
- Immunocytochemistry [1]
- Immunohistochemistry [7]
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- Product number
- UM800033 - Provider product page
- Provider
- OriGene
- Proper citation
- OriGene Cat#UM800033, RRID:AB_2629145
- Product name
- Purified KI67 (MKI67) mouse monoclonal antibody,clone UMAB107
- Antibody type
- Monoclonal
- Description
- Purified KI67 (MKI67) mouse monoclonal antibody,clone UMAB107
- Host
- Mouse
- Conjugate
- Unconjugated
- Epitope
- MKI67
- Isotype
- IgG
- Antibody clone number
- UMAB107
- Vial size
- 100 µl
- Concentration
- 1.00mg/ml
Submitted references Myoepithelial differentiation in cribriform, tubular and solid pattern of adenoid cystic carcinoma: A potential involvement in histological grading and prognosis.
Advanced prostatic ductal carcinoma in a patient with a long survival time following a total pelvis exenteration: A case report.
Primary urinary bladder adenosquamous carcinoma complicated with lower limb deep venous thromboses: a case report.
Du F, Zhou CX, Gao Y
Annals of diagnostic pathology 2016 Jun;22:12-7
Annals of diagnostic pathology 2016 Jun;22:12-7
Advanced prostatic ductal carcinoma in a patient with a long survival time following a total pelvis exenteration: A case report.
Ji C, Zhou M, Gan W, Zheng J, Yan X, Guo H
Oncology letters 2016 Feb;11(2):1509-1511
Oncology letters 2016 Feb;11(2):1509-1511
Primary urinary bladder adenosquamous carcinoma complicated with lower limb deep venous thromboses: a case report.
Zhang XL, Wei KL, Dang YW, Xie Y, Zhong TF, Ma Y, Chen G
International journal of clinical and experimental pathology 2014;7(12):9032-7
International journal of clinical and experimental pathology 2014;7(12):9032-7
No comments: Submit comment
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Western Blot analysis of HT-29 and NCI-H322M cell lysates (35ug) by using anti-KI67 monoclonal antibody (Clone UMAB107)
- Validation comment
- WB
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Western Blot analysis of MOLT-4 and MDA-MB231 cell lysates (35ug) by using anti-KI67 monoclonal antibody (Clone UMAB107)
- Validation comment
- WB
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunofluorescent staining of MDA-MB-231 cells using anti-KI67 mouse monoclonal antibody (UM800033, green, 1:100). Actin filaments were labeled with TRITC-phalloidin (red), and nuclear with DAPI (blue).
- Validation comment
- IF
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-MKI67 mouse monoclonal antibody. (UM800033; heat-induced epitope retrieval by 10mM citric buffer, pH9.0, 120C for 3min)
- Validation comment
- IHC
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded Human colon tissue using anti-MKI67 mouse monoclonal antibody. (UM800033; heat-induced epitope retrieval by 10mM citric buffer, pH9.0, 120C for 3min)
- Validation comment
- IHC
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded normal human tonsil tissue using anti-MKI67 mouse monoclonal antibody. (UM800033 at 1ug/ml for 30 min at RT; heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 20min; )
- Validation comment
- IHC
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded mouse colon tissue using anti-MKI67 mouse monoclonal antibody. (UM800033; heat-induced epitope retrieval by 10mM citric buffer, pH9.0, 120C for 3min)
- Validation comment
- IHC
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded human colon cancer tissue using anti-MKI67 mouse monoclonal antibody. (UM800033 at 1ug/ml for 30 min at RT; heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 20min; )
- Validation comment
- IHC
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded of 3 human colon cancer using anti-Ki67 clone UMAB107 mouse monoclonal antibody at 1:1000 dilution of 0.5mg/mL and detection with Polink2 Broad HRP DAB. UM800033 requires heat-induced epitope retrieval with Citrate pH6.0 in a pressure chamber/cooker high 3min. The composit image of 3 human colon cancer all show strong nuclear staining in the tumor cells.
- Validation comment
- IHC
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded of 3 human lung cancer using anti-Ki67 clone UMAB107 mouse monoclonal antibody at 1:1000 dilution of 0.5mg/mL and detection with Polink2 Broad HRP DAB. UM800033 requires heat-induced epitope retrieval with Citrate pH6.0 in a pressure chamber/cooker high 3min. The composit image of 3 human lung cancer all show strong nuclear staining in the tumor cells.
- Validation comment
- IHC
