PA5-98440

antibody from Invitrogen Antibodies

Targeting: ITSN1 ITSN, MGC134948, MGC134949, SH3D1A, SH3P17

Western blot (Supportive data in Antibodypedia)

ELISA (Recommended by provider)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-98440

Provider

Invitrogen Antibodies

Product name

ITSN1 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant full-length protein

Reactivity

Human

Host

Rabbit

Isotype

IgG

Vial size

100 µg

Concentration

3.775 mg/mL

Storage

-20°C or -80°C if preferred

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot analysis of ITSN1 using a ITSN1 Polyclonal antibody (Product # PA5-98440) at a concentration of 5 µg/mL. Positive WB detected in: 293T whole cell lysate. A secondary Goat polyclonal antibody to rabbit IgG was applied at a 1:50,000 dilution. Observed band size: 196 kDa.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot analysis of ITSN1 using a ITSN1 Polyclonal antibody (Product # PA5-98440) at a concentration of 5 µg/mL. Positive WB detected in: 293T whole cell lysate. A secondary Goat polyclonal antibody to rabbit IgG was applied at a 1:50,000 dilution. Observed band size: 196 kDa.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical analysis of ITSN1 in paraffin embedded human appendix tissue using a ITSN1 polyclonal antibody (Product # PA5-98440) at a dilution of 1:400. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.