Antibody data
- Antibody Data
- Antigen structure
- References [22]
- Comments [0]
- Validations
- Blocking/Neutralizing [1]
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- Product number
- MAB401-100 - Provider product page
- Provider
- R&D Systems
- Product name
- Mouse IL-1 beta/IL-1F2 Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from hybridoma culture supernatant. Detects mouse IL-1 beta/IL-1F2 in ELISAs.
- Reactivity
- Mouse
- Host
- Rat
- Conjugate
- Unconjugated
- Antigen sequence
P10749
- Isotype
- IgG
- Antibody clone number
- 30311
- Vial size
- 100 ug
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references Obesity-associated inflammation promotes angiogenesis and breast cancer via angiopoietin-like 4.
A20 prevents inflammasome-dependent arthritis by inhibiting macrophage necroptosis through its ZnF7 ubiquitin-binding domain.
Tumor necrosis factor family member LIGHT acts with IL-1β and TGF-β to promote airway remodeling during rhinovirus infection.
Anti-inflammatory Effects of the Natural Compounds Cortex Phellodendri and Humulus japonicus on Pelvic Inflammatory Disease in Mice.
Mucin 1 protects against severe Streptococcus pneumoniae infection.
Inhibiting the NLRP3 inflammasome with MCC950 promotes non-phlogistic clearance of amyloid-β and cognitive function in APP/PS1 mice.
Anti-inflammatory effects of a traditional Korean medicine: Ojayeonjonghwan.
Oryza sativa (Rice) Hull Extract Inhibits Lipopolysaccharide-Induced Inflammatory Response in RAW264.7 Macrophages by Suppressing Extracellular Signal-regulated Kinase, c-Jun N-terminal Kinase, and Nuclear Factor-κB Activation.
AMCase is a crucial regulator of type 2 immune responses to inhaled house dust mites.
Myeloid cell TRAF3 regulates immune responses and inhibits inflammation and tumor development in mice.
Multiple CD11c+ cells collaboratively express IL-1β to modulate stromal vascular endothelial growth factor and lymph node vascular-stromal growth.
Glimepiride reduces CD14 expression and cytokine secretion from macrophages.
Regulator of calcineurin 1 suppresses inflammation during respiratory tract infections.
Response patterns of cytokines/chemokines in two murine strains after irradiation.
NLRP10 is a NOD-like receptor essential to initiate adaptive immunity by dendritic cells.
The CDK domain of p21 is a suppressor of IL-1beta-mediated inflammation in activated macrophages.
Maternal immune activation alters fetal brain development through interleukin-6.
TNF-alpha and IL-1beta mediate inflammatory hypernociception in mice triggered by B1 but not B2 kinin receptor.
Antiinflammatory effects of natural tetranortriterpenoids isolated from Carapa guianensis Aublet on zymosan-induced arthritis in mice.
Blockade of experimental atopic dermatitis via topical NF-kappaB decoy oligonucleotide.
Sequential ELISA to profile multiple cytokines from small volumes.
Selective macrophage suppression during sepsis.
Kolb R, Kluz P, Tan ZW, Borcherding N, Bormann N, Vishwakarma A, Balcziak L, Zhu P, Davies BS, Gourronc F, Liu LZ, Ge X, Jiang BH, Gibson-Corley K, Klingelhutz A, Tan NS, Zhu Y, Sutterwala FS, Shen X, Zhang W
Oncogene 2019 Mar;38(13):2351-2363
Oncogene 2019 Mar;38(13):2351-2363
A20 prevents inflammasome-dependent arthritis by inhibiting macrophage necroptosis through its ZnF7 ubiquitin-binding domain.
Polykratis A, Martens A, Eren RO, Shirasaki Y, Yamagishi M, Yamaguchi Y, Uemura S, Miura M, Holzmann B, Kollias G, Armaka M, van Loo G, Pasparakis M
Nature cell biology 2019 Jun;21(6):731-742
Nature cell biology 2019 Jun;21(6):731-742
Tumor necrosis factor family member LIGHT acts with IL-1β and TGF-β to promote airway remodeling during rhinovirus infection.
Mehta AK, Doherty T, Broide D, Croft M
Allergy 2018 Jul;73(7):1415-1424
Allergy 2018 Jul;73(7):1415-1424
Anti-inflammatory Effects of the Natural Compounds Cortex Phellodendri and Humulus japonicus on Pelvic Inflammatory Disease in Mice.
Oh Y, Kwon YS, Jung BD
International journal of medical sciences 2017;14(8):729-734
International journal of medical sciences 2017;14(8):729-734
Mucin 1 protects against severe Streptococcus pneumoniae infection.
Dhar P, Ng GZ, Dunne EM, Sutton P
Virulence 2017 Nov 17;8(8):1631-1642
Virulence 2017 Nov 17;8(8):1631-1642
Inhibiting the NLRP3 inflammasome with MCC950 promotes non-phlogistic clearance of amyloid-β and cognitive function in APP/PS1 mice.
Dempsey C, Rubio Araiz A, Bryson KJ, Finucane O, Larkin C, Mills EL, Robertson AAB, Cooper MA, O'Neill LAJ, Lynch MA
Brain, behavior, and immunity 2017 Mar;61:306-316
Brain, behavior, and immunity 2017 Mar;61:306-316
Anti-inflammatory effects of a traditional Korean medicine: Ojayeonjonghwan.
Nam SY, Kim KY, Kim MH, Jang JB, Rah SY, Chae HJ, Lee JM, Kim HM, Jeong HJ
Pharmaceutical biology 2017 Dec;55(1):1856-1862
Pharmaceutical biology 2017 Dec;55(1):1856-1862
Oryza sativa (Rice) Hull Extract Inhibits Lipopolysaccharide-Induced Inflammatory Response in RAW264.7 Macrophages by Suppressing Extracellular Signal-regulated Kinase, c-Jun N-terminal Kinase, and Nuclear Factor-κB Activation.
Ha SK, Sung J, Choi I, Kim Y
Pharmacognosy magazine 2016 Oct-Dec;12(48):295-301
Pharmacognosy magazine 2016 Oct-Dec;12(48):295-301
AMCase is a crucial regulator of type 2 immune responses to inhaled house dust mites.
Kim LK, Morita R, Kobayashi Y, Eisenbarth SC, Lee CG, Elias J, Eynon EE, Flavell RA
Proceedings of the National Academy of Sciences of the United States of America 2015 Jun 2;112(22):E2891-9
Proceedings of the National Academy of Sciences of the United States of America 2015 Jun 2;112(22):E2891-9
Myeloid cell TRAF3 regulates immune responses and inhibits inflammation and tumor development in mice.
Lalani AI, Moore CR, Luo C, Kreider BZ, Liu Y, Morse HC 3rd, Xie P
Journal of immunology (Baltimore, Md. : 1950) 2015 Jan 1;194(1):334-48
Journal of immunology (Baltimore, Md. : 1950) 2015 Jan 1;194(1):334-48
Multiple CD11c+ cells collaboratively express IL-1β to modulate stromal vascular endothelial growth factor and lymph node vascular-stromal growth.
Benahmed F, Chyou S, Dasoveanu D, Chen J, Kumar V, Iwakura Y, Lu TT
Journal of immunology (Baltimore, Md. : 1950) 2014 May 1;192(9):4153-63
Journal of immunology (Baltimore, Md. : 1950) 2014 May 1;192(9):4153-63
Glimepiride reduces CD14 expression and cytokine secretion from macrophages.
Ingham V, Williams A, Bate C
Journal of neuroinflammation 2014 Jun 21;11:115
Journal of neuroinflammation 2014 Jun 21;11:115
Regulator of calcineurin 1 suppresses inflammation during respiratory tract infections.
Junkins RD, MacNeil AJ, Wu Z, McCormick C, Lin TJ
Journal of immunology (Baltimore, Md. : 1950) 2013 May 15;190(10):5178-86
Journal of immunology (Baltimore, Md. : 1950) 2013 May 15;190(10):5178-86
Response patterns of cytokines/chemokines in two murine strains after irradiation.
Zhang M, Yin L, Zhang K, Sun W, Yang S, Zhang B, Salzman P, Wang W, Liu C, Vidyasagar S, Zhang L, Ju S, Okunieff P, Zhang L
Cytokine 2012 May;58(2):169-77
Cytokine 2012 May;58(2):169-77
NLRP10 is a NOD-like receptor essential to initiate adaptive immunity by dendritic cells.
Eisenbarth SC, Williams A, Colegio OR, Meng H, Strowig T, Rongvaux A, Henao-Mejia J, Thaiss CA, Joly S, Gonzalez DG, Xu L, Zenewicz LA, Haberman AM, Elinav E, Kleinstein SH, Sutterwala FS, Flavell RA
Nature 2012 Apr 25;484(7395):510-3
Nature 2012 Apr 25;484(7395):510-3
The CDK domain of p21 is a suppressor of IL-1beta-mediated inflammation in activated macrophages.
Scatizzi JC, Mavers M, Hutcheson J, Young B, Shi B, Pope RM, Ruderman EM, Samways DS, Corbett JA, Egan TM, Perlman H
European journal of immunology 2009 Mar;39(3):820-5
European journal of immunology 2009 Mar;39(3):820-5
Maternal immune activation alters fetal brain development through interleukin-6.
Smith SE, Li J, Garbett K, Mirnics K, Patterson PH
The Journal of neuroscience : the official journal of the Society for Neuroscience 2007 Oct 3;27(40):10695-702
The Journal of neuroscience : the official journal of the Society for Neuroscience 2007 Oct 3;27(40):10695-702
TNF-alpha and IL-1beta mediate inflammatory hypernociception in mice triggered by B1 but not B2 kinin receptor.
Cunha TM, Verri WA Jr, Fukada SY, Guerrero AT, Santodomingo-Garzón T, Poole S, Parada CA, Ferreira SH, Cunha FQ
European journal of pharmacology 2007 Nov 14;573(1-3):221-9
European journal of pharmacology 2007 Nov 14;573(1-3):221-9
Antiinflammatory effects of natural tetranortriterpenoids isolated from Carapa guianensis Aublet on zymosan-induced arthritis in mice.
Penido C, Conte FP, Chagas MS, Rodrigues CA, Pereira JF, Henriques MG
Inflammation research : official journal of the European Histamine Research Society ... [et al.] 2006 Nov;55(11):457-64
Inflammation research : official journal of the European Histamine Research Society ... [et al.] 2006 Nov;55(11):457-64
Blockade of experimental atopic dermatitis via topical NF-kappaB decoy oligonucleotide.
Dajee M, Muchamuel T, Schryver B, Oo A, Alleman-Sposeto J, De Vry CG, Prasad S, Ruhrmund D, Shyamsundar R, Mutnick D, Mai K, Le T, Parham C, Zhang J, Komuves L, Colby T, Hudak S, McEvoy LM, Ehrhardt RO
The Journal of investigative dermatology 2006 Aug;126(8):1792-803
The Journal of investigative dermatology 2006 Aug;126(8):1792-803
Sequential ELISA to profile multiple cytokines from small volumes.
Osuchowski MF, Siddiqui J, Copeland S, Remick DG
Journal of immunological methods 2005 Jul;302(1-2):172-81
Journal of immunological methods 2005 Jul;302(1-2):172-81
Selective macrophage suppression during sepsis.
Ellaban E, Bolgos G, Remick D
Cellular immunology 2004 Sep-Oct;231(1-2):103-11
Cellular immunology 2004 Sep-Oct;231(1-2):103-11
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Supportive validation
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- Experimental details
- Cell Proliferation Induced by IL-1 beta/IL-1F2 and Neutral-ization by Mouse IL-1 beta/IL-1F2 Antibody. Recombinant Mouse IL-1 beta/IL-1F2 (Catalog # 401-ML) stimulates proliferation in the the D10.G4.1 mouse helper T cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Mouse IL-1 beta/IL-1F2 (50 pg/mL) is neutralized (green line) by increasing concentrations of Rat Anti-Mouse IL-1 beta/IL-1F2 Monoclonal Antibody (Catalog # MAB401). The ND50 is typically 1-5 µg/mL in the presence of concanavalin A (1.25 µg/mL).