Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [3]
- Immunohistochemistry [1]
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Validation data
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- Product number
- LS-B3455 - Provider product page
- Provider
- LSBio
- Proper citation
- LifeSpan Cat#LS-B3455, RRID:AB_2144883
- Product name
- IHC-plus™ MMP9 / Gelatinase B Antibody (aa644-673) LS-B3455
- Antibody type
- Polyclonal
- Description
- Ammonium sulfate precipitation
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Storage
- Maintain refrigerated at 2°C to 8°C for up to 6 months. For long term storage store at -20°C.
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Supportive validation
- Submitted by
- LSBio (provider)
- Main image
- Experimental details
- Western blot of lysate from NCI-H1299 cell line, using MMP9 Antibody (L659). Antibody was diluted at 1:1000. A goat anti-rabbit IgG H&L (HRP) at 1:10000 dilution was used as the secondary antibody. Lysate at 35ug.
- Submitted by
- LSBio (provider)
- Main image
- Experimental details
- MMP9 Antibody western blot of 293 cell line lysates (35 ug/lane). The MMP9 antibody detected the MMP9 protein (arrow).
- Submitted by
- LSBio (provider)
- Main image
- Experimental details
- Western blot of MMP9 (arrow) in mouse spleen lysates (35 ug/lane). MMP9 (arrow) was detected using the purified antibody.
Supportive validation
- Submitted by
- LSBio (provider)
- Main image
- Experimental details
- Anti-MMP-9 antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.