Explore
Validate
Learn
Western blot
ImmunohistochemistryAntibody data
- Antibody Data
- Antigen structure
- References [3]
- Comments [0]
- Validations
- Western blot [7]
- Immunohistochemistry [3]
Submit
Validation data
Reference
Comment
Report error
- Product number
- GTX108592 - Provider product page
- Provider
- GeneTex
- Proper citation
- GeneTex Cat#GTX108592, RRID:AB_1952260
- Product name
- ZO-1 antibody
- Antibody type
- Polyclonal
- Reactivity
- Human, Mouse
- Host
- Rabbit
Submitted references The FOXN3-NEAT1-SIN3A repressor complex promotes progression of hormonally responsive breast cancer.
Oxyresveratrol improves tight junction integrity through the PKC and MAPK signaling pathways in Caco-2 cells.
Conditioned medium from LS 174T goblet cells treated with oxyresveratrol strengthens tight junctions in Caco-2 cells.
Li W, Zhang Z, Liu X, Cheng X, Zhang Y, Han X, Zhang Y, Liu S, Yang J, Xu B, He L, Sun L, Liang J, Shang Y
The Journal of clinical investigation 2017 Sep 1;127(9):3421-3440
The Journal of clinical investigation 2017 Sep 1;127(9):3421-3440
Oxyresveratrol improves tight junction integrity through the PKC and MAPK signaling pathways in Caco-2 cells.
Jo H, Hwang D, Kim JK, Lim YH
Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association 2017 Oct;108(Pt A):203-213
Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association 2017 Oct;108(Pt A):203-213
Conditioned medium from LS 174T goblet cells treated with oxyresveratrol strengthens tight junctions in Caco-2 cells.
Hwang D, Jo H, Hwang S, Kim JK, Kim IH, Lim YH
Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie 2017 Jan;85:280-286
Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie 2017 Jan;85:280-286
No comments: Submit comment
Enhanced validation
Supportive validation
- Submitted by
- GeneTex (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- Non-transfected (¡V) and transfected (+) HeLa whole cell extracts (30 ?g) were separated by 5% SDS-PAGE, and the membrane was blotted with ZO-1 antibody (GTX108592) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Sample (30 ug of whole cell lysate)A: H1299 7.5% SDS PAGEGTX108592 diluted at 1:500
- Validation comment
- WB
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- ZO-1 antibody detects ZO-1 protein by western blot analysis. Various whole cell extracts (30 ?g) were separated by 5% SDS-PAGE, and the membrane was blotted with ZO-1 antibody (GTX108592) diluted by 1:500.
- Validation comment
- WB
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- ZO-1 antibody detects ZO-1 protein by western blot analysis. Various whole cell extracts (30 ?g) were separated by 5% SDS-PAGE, and the membrane was blotted with ZO-1 antibody (GTX108592) diluted by 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- ZO-1 antibody detects ZO-1 protein by western blot analysis. Various whole cell extracts (30 ?g) were separated by 5% SDS-PAGE, and the membrane was blotted with ZO-1 antibody (GTX108592) diluted by 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Various whole cell extracts (30 ?g) were separated by 5% SDS-PAGE, and the membrane was blotted with ZO-1 antibody (GTX108592) diluted at 1:1000.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Non-transfected (¡V) and transfected (+) HeLa whole cell extracts (30 ?g) were separated by 5% SDS-PAGE, and the membrane was blotted with ZO-1 antibody (GTX108592) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded Mouse colon, using ZO-1(GTX108592) antibody at 1:100 dilution. [Image courtesy of Sergei grivennikov (Laboratory of?Michael Karin, UCSD).]
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- ZO-1 antibody detects ZO-1 protein at cell membrane and cytoplasm in mouse testis by immunohistochemical analysis. Sample: Paraffin-embedded mouse testis. ZO-1 antibody (GTX108592) diluted at 1:500.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- ZO-1 antibody detects ZO-1 protein at cell membrane and cytoplasm in mouse cervix by immunohistochemical analysis. Sample: Paraffin-embedded mouse cervix. ZO-1 antibody (GTX108592) diluted at 1:500.
