GTX108613

antibody from GeneTex

Targeting: TJP1 DKFZp686M05161, MGC133289, ZO-1

Western blot (Enhanced validation)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

GTX108613

Provider

GeneTex

Proper citation

GeneTex Cat#GTX108613, RRID:AB_1952257

Product name

ZO-1 antibody [N1N2], N-term

Antibody type

Polyclonal

Reactivity

Human, Mouse

Host

Rabbit

References

Submitted references

Astragaloside IV protects blood-brain barrier integrity from LPS-induced disruption via activating Nrf2 antioxidant signaling pathway in mice.

Li H, Wang P, Huang F, Jin J, Wu H, Zhang B, Wang Z, Shi H, Wu X
Toxicology and applied pharmacology 2018 Feb 1;340:58-66

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SLC26A3 (DRA) prevents TNF-alpha-induced barrier dysfunction and dextran sulfate sodium-induced acute colitis.

Ding X, Li D, Li M, Wang H, He Q, Wang Y, Yu H, Tian D, Yu Q
Laboratory investigation; a journal of technical methods and pathology 2018 Apr;98(4):462-476

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Neutralization of IL-8 decreases tumor PMN-MDSCs and reduces mesenchymalization of claudin-low triple-negative breast cancer.

Dominguez C, McCampbell KK, David JM, Palena C
JCI insight 2017 Nov 2;2(21)

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Helicobacter pylori CagA and IL-1β Promote the Epithelial-to-Mesenchymal Transition in a Nontransformed Epithelial Cell Model.

Arévalo-Romero H, Meza I, Vallejo-Flores G, Fuentes-Pananá EM
Gastroenterology research and practice 2016;2016:4969163

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The Synthetic β-Nitrostyrene Derivative CYT-Rx20 Inhibits Esophageal Tumor Growth and Metastasis via PI3K/AKT and STAT3 Pathways.

Chiu WC, Lee YC, Su YH, Wang YY, Tsai CH, Hou YA, Wang CH, Huang YF, Huang CJ, Chou SH, Hsieh PW, Yuan SF
PloS one 2016;11(11):e0166453

Western blot

Enhanced validation

Submitted by

GeneTex (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Non-transfected (¡V) and transfected (+) HeLa whole cell extracts (30 ?g) were separated by 5% SDS-PAGE, and the membrane was blotted with ZO-1 antibody [N1N2], N-term (GTX108613) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

KD Reagent Type

siRNA

Downregulation

>75%

KD Reagent Prod page

KD Reagent Prod page

KD Reagent Provider

shRNA reagents was provided courtesy of cooperative labs.

KD Reagent Prod no

Proprietary.

Antibody Lot Number

39967

Show more

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Sample(30 ?g whole cell lysate)A:H12995% SDS PAGEGTX108613 diluted at 1:3000The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Mouse tissue extract (50 ?g) was separated by 5% SDS-PAGE, and the membrane was blotted with ZO-1 antibody [N1N2], N-term (GTX108613) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Various whole cell extracts (30 ?g) were separated by 5% SDS-PAGE, and the membrane was blotted with ZO-1 antibody [N1N2], N-term (GTX108613) diluted at 1:1000.

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Various whole cell extracts (30 ?g) were separated by 5% SDS-PAGE, and the membrane was blotted with ZO-1 antibody [N1N2], N-term (GTX108613) diluted at 1:3000.

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Non-transfected (¡V) and transfected (+) HeLa whole cell extracts (30 ?g) were separated by 5% SDS-PAGE, and the membrane was blotted with ZO-1 antibody [N1N2], N-term (GTX108613) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Immunocytochemistry

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

ZO-1 antibody [N1N2], N-term detects TJP1 protein at junction by confocal immunofluorescent analysis. Sample: A431 cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: TJP1 protein stained by ZO-1 antibody [N1N2], N-term (GTX108613) diluted at 1:500.Blue: Hoechst 33342 staining.[Images captured by Olympus FV10i Confocal Laser Scanning Microscope]

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

ZO-1 antibody [N1N2], N-term detects ZO-1 protein at cell membrane by immunofluorescent analysis.Sample: NT2D1 cells were fixed in ice-cold MeOH for 5 min.Green: ZO-1 protein stained by ZO-1 antibody [N1N2], N-term (GTX108613) diluted at 1:200.Blue: Hoechst 33342 staining.Scale bar = 10 £gm.

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

ZO-1 antibody [N1N2], N-term detects ZO-1 protein at cell membrane by immunofluorescent analysis.Sample: NT2D1 cells were fixed in ice-cold MeOH for 5 min.Green: ZO-1 stained by ZO-1 antibody [N1N2], N-term (GTX108613) diluted at 1:200.Blue: Hoechst 33342 staining.

Immunohistochemistry

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Immunohistochemical analysis of paraffin-embedded H661 xenograft, using ZO-1(GTX108613) antibody at 1:250 dilution.

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

ZO-1 antibody [N1N2], N-term detects ZO-1 protein at cell membrane and cytoplasm in mouse testis by immunohistochemical analysis. Sample: Paraffin-embedded mouse testis. ZO-1 antibody [N1N2], N-term (GTX108613) diluted at 1:500.

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

ZO-1 antibody [N1N2], N-term detects ZO-1 protein at cell membrane and cytoplasm in mouse intestine by immunohistochemical analysis. Sample: Paraffin-embedded mouse intestine. ZO-1 antibody [N1N2], N-term (GTX108613) diluted at 1:500.