abx000800

antibody from Abbexa

Targeting: TJP1 DKFZp686M05161, MGC133289, ZO-1

Western blot (Recommended by provider)

Antibody Data

Product number

abx000800

Provider

Abbexa

Product name

Tight Junction Protein ZO-1 (TJP1) Antibody

Antibody type

Polyclonal

Description

TJP1 Antibody is a Rabbit Polyclonal antibody against TJP1 (ZO1). Tight junctions, or zona occludens, form a continuous barrier to fluids across the epithelium and endothelium. They function in regulation of paracellular permeability and in the maintenance of cell polarity, blocking the movement of transmembrane proteins between the apical and the basolateral cell surfaces (reviewed in 1). Zona occludens proteins ZO-1, -2, and -3 (also known as TJP1, 2, and 3) are peripheral membrane adaptor proteins that link junctional transmembrane proteins such as occludin and claudin to the actin cytoskeleton (reviewed in 2). ZO-1 and -2 are required for tight junction formation and function (3,4). In subconfluent proliferating cells, ZO-1 and ZO-2 have been shown to colocalize to the nucleus and play a role in transcriptional regulation, possibly through facilitating nuclear import/export of transcriptional regulators (5-7). The ZO-2 gene is transcribed from two promoters, generating the ZO-2A and ZO-2C isoforms. ZO-2C lacks a 23 amino acid amino-terminal sequence found in other ZO-2 isoforms. While both isoforms appear to be widely expressed, abnormal regulation of the ZO-2 gene may be correlated with development of ductal cancer (8).

Reactivity

Human, Mouse

Host

Rabbit

Conjugate

Unconjugated

Isotype

IgG

Vial size

100 µl

Concentration

1.26 mg/ml

Storage

Aliquot and store at -20°C. Avoid repeated freeze/thaw cycles.