M00860

antibody from Boster Biological Technology

Targeting: TJP1 DKFZp686M05161, MGC133289, ZO-1

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Data presented on provider website)

Immunohistochemistry (Data presented on provider website)

Flow cytometry (Data presented on provider website)

Antibody Data

Product number

M00860

Provider

Boster Biological Technology

Product name

Anti-TJP1 Antibody Picoband™ (monoclonal, 3E12)

Antibody type

Monoclonal

Description

Mouse IgG monoclonal antibody for TJP1 detection. Tested with WB, IHC-P, ICC/IF, FCM in Human.

Reactivity

Human

Host

Mouse

Isotype

IgG

Antibody clone number

3.00E+12

Vial size

100μg/vial

Storage

At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquoted and stored frozen at -20°C for a longer time. Avoid repeated freezing and thawing.

Handling

Add 0.2ml of distilled water will yield a concentration of 500μg/ml.

Western blot

Supportive validation

Submitted by

Boster Biological Technology (provider)

Main image

Experimental details

Western blot analysis of ZO-1 using anti ZO-1 antibody (M00860). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human Caco-2 tissue lysates, Lane 2: human U2OS whole cell lysates, Lane 3: human K562 whole cell lysates, Lane 4: human PC-3 whole cell lysates, Lane 5: human T-47D whole cell lysates, After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-ZO-1 antigen affinity purified polyclonal antibody (Catalog # M00860) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001) with Tanon 5200 system. A specific band was detected for ZO-1 at approximately 240KD. The expected band size for ZO-1 is at 195KD.

Additional image