Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [3]
- Flow cytometry [1]
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Validation data
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- Product number
- R31450 - Provider product page
- Provider
- NSJ Bioreagents
- Product name
- ICAM-1 Antibody
- Antibody type
- Polyclonal
- Antigen
- Human partial recombinant protein (AA 214-532) was used as the immunogen for this ICAM-1 antibody.
- Description
- Antigen affinity purified antibody
- Reactivity
- Human
- Host
- Rabbit
- Conjugate
- Unconjugated
- Vial size
- 100 µg
- Concentration
- Lyophilized; resuspend with 200 ul for 0.5 mg/ml
- Storage
- After reconstitution, the ICAM-1 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.
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Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Western blot testing of ICAM-1 antibody and Lane 1: HEPG2; 2: K562; 3: A549; 4: HT1080 lysate. The glycoprotein is routinely visualized at 75~115KD.
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- IHC-P: ICAM-1 antibody testing of human intestinal cancer tissue
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Immunofluorescent staining of FFPE human tonsil tissue with ICAM-1 antibody (green) at 1ug/ml and DAPI nuclear counterstain (blue). HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min and allow to cool before testing.
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Immunofluorescent staining of FFPE human lung cancer with ICAM-1 antibody (green) at 1ug/ml and DAPI nuclear counterstain (blue). HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min and allow to cool before testing.
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Flow cytometry testing of human A431 cells with ICAM-1 antibody at 1ug/10^6 cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= ICAM-1 antibody.