Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunohistochemistry [1]
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Validation data
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- Product number
- PA5-21778 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- ICAM-2 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: 293T, A431, H1299, HeLaS3, HepG2, Molt-4, Raji. Predicted reactivity: Rhesus Monkey (84%), Chimpanzee (99%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using ICAM-2 Polyclonal Antibody (Product # PA5-21778). Sample (30 µg whole cell lysate). A: HeLa S3. 12% SDS PAGE. ICAM-2 Polyclonal Antibody (Product # PA5-21778) diluted at 1:1,000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot was performed using Anti-ICAM-2 Polyclonal Antibody (Product # PA5-21778) and a 31 kDa band corresponding to ICAM-2 was observed across cell lines and tissue extracts tested. Due to glycosylation a band around 60 kDa has been observed in Jurkat. Membrane enriched extracts (30 µg lysate) of Jurkat (Lane 1), K-562 (Lane 2), HL-60 (Lane 3), MOLT-4 (Lane 4), tissue extracts of Mouse Lung (Lane 5), Mouse Spleen (Lane 6) and Mouse Thymus (Lane 7) were electrophoresed using NuPAGE™ 4-12% Bis-Tris Protein Gel (Product # NP0322BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:1000 dilution) and detected by chemiluminescence with Goat anti-Rabbit IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using SuperSignal™ West Dura Extended Duration Substrate (Product # 34076).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded human breast cancerncer, using ICAM2 (Product # PA5-21778) antibody at 1:100 dilution. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.