R4528

antibody from MilliporeSigma / Merck KGaA

Targeting: HNRNPA1 ALS20, hnRNP-A1, HNRPA1

Western blot (Recommended by provider)

Immunocytochemistry (Recommended by provider)

Immunoprecipitation (Recommended by provider)

Immunohistochemistry (Recommended by provider)

Antibody Data

Product number

R4528

Provider

MilliporeSigma / Merck KGaA

Product name

Anti-hnRNP-A1 antibody, Mouse monoclonal

Antibody type

Monoclonal

Reactivity

Human, Mouse, Rat, Bovine, Canine, Hamster, Simian

Host

Mouse

Conjugate

Unconjugated

Isotype

IgG

Antibody clone number

9H10

Storage

Store at -20°C

Western blot

Supportive validation

Submitted by

per

Main image

Experimental details

Western blot analysis of antibody specificity using a routine panel composed of IgG/HSA-depleted human plasma and protein lysates from selected human tissues and cell lines.

Validation comment

Band of predicted size in kDa (+/-20%) with additional bands present.

Primary Ab dilution

1:500

Secondary Ab dilution

1:7000

Lane 1

Marker [kDa]: 219, 111, 83, 48, 32, 26, 17

Lane 2

RT-4

Lane 3

U-251MG sp

Lane 4

Human Plasma

Lane 5

Liver

Lane 6

Tonsil

Theoretical target weight

[kDa] 34

Immunohistochemistry

Supportive validation

Submitted by

per

Main image

Experimental details

Immunohistochemical staining of human rectum shows strong nuclear positivity in glandular cells.

Validation comment

Two independent antibodies targeting one protein yielding similar staining patterns. Staining pattern consistent with experimental and/or bioinformatic data.