Antibody data
- Antibody Data
- Antigen structure
- References [18]
- Comments [0]
- Validations
- Flow cytometry [1]
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- Product number
- Q10013 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- CD14 Monoclonal Antibody (TüK4), Qdot™ 605
- Antibody type
- Monoclonal
- Antigen
- Other
- Description
- Qdot™ Antibody (Ab) conjugates possess a bright fluorescence emission that makes them well suited for the detection of low-abundance extracellular proteins. Approximately the same size as R-phycoerythrin (R-PE) and compatible with existing organic fluorophore conjugates, Qdot™ Ab conjugates can be excited with any wavelength below their emission maximum, but are best excited by UV or violet light. The narrow, symmetric emission profiles of Qdot Ab conjugates allow for minimal compensation when using a single excitation source, and the very long stoke shifts enable better, more efficient multicolor assays using the 405 nm violet laser. Available in multiple colors for use in flow cytometry, these advantages make Qdot Ab conjugates powerful tools for antibody labeling and staining. Staining: Stain cells in any standard staining buffer, such as phosphate buffered saline (PBS) with 1% bovine serum albumin (BSA). We recommend analysis of cells within 18 hours of staining. If dilute reagent is used, dilute only the quantity of reagent to be used within one day. Qdot Ab conjugates may be mixed with other antibodies, but use the diluted conjugates on the day of dilution. Qdot Ab conjugates can be used for surface staining applications with most conventional sample preparation reagents, such as Cal-Lyse™ Lysing Solution and FIX & PERM™ reagents, with minimal effect on fluorescence. We have observed some batches of BD FACS™ Lysing Solution to interfere with Qdot Ab conjugate fluorescence. Each lot has been tested by flow cytometry using human peripheral blood leukocytes. The isotype control for this antibody is mouse IgG2a, Cat. No. Q10014. Instrument setup: Qdot Ab conjugates are excited optimally with UV or 405 nm light, although excitation can be obtained with any wavelength below the emission maximum of a given Qdot™ nanocrystal, such as with a 488-nm laser. Qdot Ab conjugates can be used on cytometers that do not have UV or violet excitation sources as long as they have appropriate emission filters. Make sure the cytometer has an appropriate emission filter for the Qdot Ab conjugate being used; alternate filters can be used as long as they capture the emission maximum, but filter width impacts spectral overlap corrections. And be sure to check for Qdot Ab conjugate emission in any channel that can capture nanocrystal emission off of other lasers on the cytometer. For Cat. No. Q10013: peak excitation 405 (488) nm/peak emission 605 nm; recommended filter 605/20 nm. Store reagents at 2-8°C in the dark. Do not freeze. Because Qdot nanocrystals are conjugated to biological materials, some loss of activity may be observed with prolonged storage. When stored as instructed, expires six months from date of receipt unless otherwise indicated on product label. Qdot Ab conjugates are photostable, and do not need to be protected from light. However, if using Qdot Ab conjugates in combination with conventional fluorochrome conjugated antibodies, minimize light exposure during handling, incubation with cells, and prior to analysis. The Qdot Ab conjugates contain cadmium and selenium in an inorganic crystalline form. Dispose of the material in compliance with all applicable local, state, and federal regulations for disposal of these classes of material. For more information on the composition of these materials, consult the Safety Data Sheets (SDSs).
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- TüK4
- Vial size
- 100 µL
- Storage
- 4° C
Submitted references E4orf1 Suppresses E1B-Deleted Adenovirus Vaccine-Induced Immune Responses.
Fibroblast Heterogeneity and Immunosuppressive Environment in Human Breast Cancer.
Metabolic Phenotypes of Response to Vaccination in Humans.
Adjuvanting a Simian Immunodeficiency Virus Vaccine with Toll-Like Receptor Ligands Encapsulated in Nanoparticles Induces Persistent Antibody Responses and Enhanced Protection in TRIM5α Restrictive Macaques.
Effect of chronic morphine administration on circulating dendritic cells in SIV-infected rhesus macaques.
Tissue factor expression by myeloid cells contributes to protective immune response against Mycobacterium tuberculosis infection.
Vaccine Induction of Lymph Node-Resident Simian Immunodeficiency Virus Env-Specific T Follicular Helper Cells in Rhesus Macaques.
An Fc engineering approach that modulates antibody-dependent cytokine release without altering cell-killing functions.
Efficacy and safety of CDX-301, recombinant human Flt3L, at expanding dendritic cells and hematopoietic stem cells in healthy human volunteers.
Differential leukocyte counting and immunophenotyping in cryopreserved ex vivo whole blood.
Differential regulatory T cell activity in HIV type 1-exposed seronegative individuals.
Sequential staining improves detection of CCR2 and CX3CR1 on monocytes when simultaneously evaluating CCR5 by multicolor flow cytometry.
Age-related patterns in human myeloid dendritic cell populations in people exposed to Schistosoma haematobium infection.
Optimization of a whole blood intracellular cytokine assay for measuring innate cell responses to mycobacteria.
Variations in the heme oxygenase-1 microsatellite polymorphism are associated with plasma CD14 and viral load in HIV-infected African-Americans.
A CD8α(-) subpopulation of macaque circulatory natural killer cells can mediate both antibody-dependent and antibody-independent cytotoxic activities.
The human syndrome of dendritic cell, monocyte, B and NK lymphoid deficiency.
Quantum dots thermal stability improves simultaneous phenotype-specific telomere length measurement by FISH-flow cytometry.
Sangare K, Helmold Hait S, Moore M, Hogge C, Hoang T, Rahman MA, Venzon DJ, LaBranche C, Montefiori D, Robert-Guroff M, Thomas MA
Vaccines 2022 Feb 15;10(2)
Vaccines 2022 Feb 15;10(2)
Fibroblast Heterogeneity and Immunosuppressive Environment in Human Breast Cancer.
Costa A, Kieffer Y, Scholer-Dahirel A, Pelon F, Bourachot B, Cardon M, Sirven P, Magagna I, Fuhrmann L, Bernard C, Bonneau C, Kondratova M, Kuperstein I, Zinovyev A, Givel AM, Parrini MC, Soumelis V, Vincent-Salomon A, Mechta-Grigoriou F
Cancer cell 2018 Mar 12;33(3):463-479.e10
Cancer cell 2018 Mar 12;33(3):463-479.e10
Metabolic Phenotypes of Response to Vaccination in Humans.
Li S, Sullivan NL, Rouphael N, Yu T, Banton S, Maddur MS, McCausland M, Chiu C, Canniff J, Dubey S, Liu K, Tran V, Hagan T, Duraisingham S, Wieland A, Mehta AK, Whitaker JA, Subramaniam S, Jones DP, Sette A, Vora K, Weinberg A, Mulligan MJ, Nakaya HI, Levin M, Ahmed R, Pulendran B
Cell 2017 May 18;169(5):862-877.e17
Cell 2017 May 18;169(5):862-877.e17
Adjuvanting a Simian Immunodeficiency Virus Vaccine with Toll-Like Receptor Ligands Encapsulated in Nanoparticles Induces Persistent Antibody Responses and Enhanced Protection in TRIM5α Restrictive Macaques.
Kasturi SP, Kozlowski PA, Nakaya HI, Burger MC, Russo P, Pham M, Kovalenkov Y, Silveira ELV, Havenar-Daughton C, Burton SL, Kilgore KM, Johnson MJ, Nabi R, Legere T, Sher ZJ, Chen X, Amara RR, Hunter E, Bosinger SE, Spearman P, Crotty S, Villinger F, Derdeyn CA, Wrammert J, Pulendran B
Journal of virology 2017 Feb 15;91(4)
Journal of virology 2017 Feb 15;91(4)
Effect of chronic morphine administration on circulating dendritic cells in SIV-infected rhesus macaques.
Cornwell WD, Wagner W, Lewis MG, Fan X, Rappaport J, Rogers TJ
Journal of neuroimmunology 2016 Jun 15;295-296:30-40
Journal of neuroimmunology 2016 Jun 15;295-296:30-40
Tissue factor expression by myeloid cells contributes to protective immune response against Mycobacterium tuberculosis infection.
Venkatasubramanian S, Tripathi D, Tucker T, Paidipally P, Cheekatla S, Welch E, Raghunath A, Jeffers A, Tvinnereim AR, Schechter ME, Andrade BB, Mackman N, Idell S, Vankayalapati R
European journal of immunology 2016 Feb;46(2):464-79
European journal of immunology 2016 Feb;46(2):464-79
Vaccine Induction of Lymph Node-Resident Simian Immunodeficiency Virus Env-Specific T Follicular Helper Cells in Rhesus Macaques.
Vargas-Inchaustegui DA, Demers A, Shaw JM, Kang G, Ball D, Tuero I, Musich T, Mohanram V, Demberg T, Karpova TS, Li Q, Robert-Guroff M
Journal of immunology (Baltimore, Md. : 1950) 2016 Feb 15;196(4):1700-10
Journal of immunology (Baltimore, Md. : 1950) 2016 Feb 15;196(4):1700-10
An Fc engineering approach that modulates antibody-dependent cytokine release without altering cell-killing functions.
Kinder M, Greenplate AR, Strohl WR, Jordan RE, Brezski RJ
mAbs 2015;7(3):494-504
mAbs 2015;7(3):494-504
Efficacy and safety of CDX-301, recombinant human Flt3L, at expanding dendritic cells and hematopoietic stem cells in healthy human volunteers.
Anandasabapathy N, Breton G, Hurley A, Caskey M, Trumpfheller C, Sarma P, Pring J, Pack M, Buckley N, Matei I, Lyden D, Green J, Hawthorne T, Marsh HC, Yellin M, Davis T, Keler T, Schlesinger SJ
Bone marrow transplantation 2015 Jul;50(7):924-30
Bone marrow transplantation 2015 Jul;50(7):924-30
Differential leukocyte counting and immunophenotyping in cryopreserved ex vivo whole blood.
Nemes E, Kagina BM, Smit E, Africa H, Steyn M, Hanekom WA, Scriba TJ
Cytometry. Part A : the journal of the International Society for Analytical Cytology 2015 Feb;87(2):157-65
Cytometry. Part A : the journal of the International Society for Analytical Cytology 2015 Feb;87(2):157-65
Differential regulatory T cell activity in HIV type 1-exposed seronegative individuals.
Pattacini L, Murnane PM, Kahle EM, Bolton MJ, Delrow JJ, Lingappa JR, Katabira E, Donnell D, McElrath MJ, Baeten JM, Lund JM
AIDS research and human retroviruses 2013 Oct;29(10):1321-9
AIDS research and human retroviruses 2013 Oct;29(10):1321-9
Sequential staining improves detection of CCR2 and CX3CR1 on monocytes when simultaneously evaluating CCR5 by multicolor flow cytometry.
Jalbert E, Shikuma CM, Ndhlovu LC, Barbour JD
Cytometry. Part A : the journal of the International Society for Analytical Cytology 2013 Mar;83(3):280-6
Cytometry. Part A : the journal of the International Society for Analytical Cytology 2013 Mar;83(3):280-6
Age-related patterns in human myeloid dendritic cell populations in people exposed to Schistosoma haematobium infection.
Nausch N, Louis D, Lantz O, Peguillet I, Trottein F, Chen IY, Appleby LJ, Bourke CD, Midzi N, Mduluza T, Mutapi F
PLoS neglected tropical diseases 2012;6(9):e1824
PLoS neglected tropical diseases 2012;6(9):e1824
Optimization of a whole blood intracellular cytokine assay for measuring innate cell responses to mycobacteria.
Shey MS, Hughes EJ, de Kock M, Barnard C, Stone L, Kollmann TR, Hanekom WA, Scriba TJ
Journal of immunological methods 2012 Feb 28;376(1-2):79-88
Journal of immunological methods 2012 Feb 28;376(1-2):79-88
Variations in the heme oxygenase-1 microsatellite polymorphism are associated with plasma CD14 and viral load in HIV-infected African-Americans.
Seu L, Burt TD, Witte JS, Martin JN, Deeks SG, McCune JM
Genes and immunity 2012 Apr;13(3):258-67
Genes and immunity 2012 Apr;13(3):258-67
A CD8α(-) subpopulation of macaque circulatory natural killer cells can mediate both antibody-dependent and antibody-independent cytotoxic activities.
Vargas-Inchaustegui DA, Demberg T, Robert-Guroff M
Immunology 2011 Nov;134(3):326-40
Immunology 2011 Nov;134(3):326-40
The human syndrome of dendritic cell, monocyte, B and NK lymphoid deficiency.
Bigley V, Haniffa M, Doulatov S, Wang XN, Dickinson R, McGovern N, Jardine L, Pagan S, Dimmick I, Chua I, Wallis J, Lordan J, Morgan C, Kumararatne DS, Doffinger R, van der Burg M, van Dongen J, Cant A, Dick JE, Hambleton S, Collin M
The Journal of experimental medicine 2011 Feb 14;208(2):227-34
The Journal of experimental medicine 2011 Feb 14;208(2):227-34
Quantum dots thermal stability improves simultaneous phenotype-specific telomere length measurement by FISH-flow cytometry.
Kapoor V, Hakim FT, Rehman N, Gress RE, Telford WG
Journal of immunological methods 2009 May 15;344(1):6-14
Journal of immunological methods 2009 May 15;344(1):6-14
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Supportive validation
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- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Qdot® 605 anti-human CD14 conjugate 605/20 band pass filter