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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [1]
- Immunohistochemistry [2]
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Validation data
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- Product number
- PAB0274 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#PAB0274, RRID:AB_1019072
- Product name
- TRAF1 polyclonal antibody
- Antibody type
- Polyclonal
- Description
- Rabbit polyclonal antibody raised against synthetic peptide of TRAF1.
- Antigen sequence
MASSSGSSPRPAPDENEFPFG- Storage
- Store at -20°C.Aliquot to avoid repeated freezing and thawing.
Submitted references TNFR-associated factor family protein expression in normal tissues and lymphoid malignancies.
Zapata JM, Krajewska M, Krajewski S, Kitada S, Welsh K, Monks A, McCloskey N, Gordon J, Kipps TJ, Gascoyne RD, Shabaik A, Reed JC
Journal of immunology (Baltimore, Md. : 1950) 2000 Nov 1;165(9):5084-96
Journal of immunology (Baltimore, Md. : 1950) 2000 Nov 1;165(9):5084-96
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Western blot analysis of TRAF1 in normal human tissues. Using TRAF1 polyclonal antibody (Cat # PAB0274) at 1 : 2000. TRAF1 is observed at 50-50 KDa. Additional bands of lower molecular weight were seen in some cases, and may represent TRAF1 degradation fragments.
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Formalin-fixed, paraffin-embedded human lymphoma tissue section stained for TRAF1 expression. Using TRAF1 polyclonal antibody (Cat # PAB0274) at 1 : 2000. Hematoxylin-eosin counterstain. A1 is a higher magnification of A.
- Validation comment
- Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Formalin-fixed, paraffin-embedded human tissue sections stained for TRAF1 expression. Using TRAF1 polyclonal antibody (Cat # PAB0274) at 1 : 2000. Hematoxylin-eosin counterstain. A, A1 : Formalin-fixed, paraffin-embedded human lymphoma tissue section stained for TRAF1 expression. Using TRAF1 polyclonal antibody (Cat # PAB0274) at 1 : 2000. Hematoxylin-eosin counterstain. A, Hodgkin's Disease, the Reed-Sternberg cells are positive for TRAF1 expression. B, lymph node, dendritic-like cells are positive for TRAF1 expression. A1 and B1 are higher magnifications from A and B, respectively. .
- Validation comment
- Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
