HPA003648

antibody from Atlas Antibodies

Targeting: GPR143 OA1

Immunohistochemistry (Enhanced validation)

Antibody Data

Product number

HPA003648

Provider

Atlas Antibodies

Proper citation

Atlas Antibodies Cat#HPA003648, RRID:AB_2667028

Product name

Anti-GPR143

Antibody type

Polyclonal

Reactivity

Human

Host

Rabbit

Conjugate

Unconjugated

Antigen sequence

CSLGFQSPRKEIQWESLTTSAAEGAHPSPLMPHEN
PASGKVSQVGGQTSDEALSMLSEGSDASTIEIHTA
SESCNKNEGDPALPT


Isotype

IgG

Vial size

100 µl

Storage

Store at +4°C for short term storage. Long time storage is recommended at -20°C.

Immunohistochemistry

Enhanced validation

Submitted by

Atlas Antibodies (provider)

Enhanced method

Orthogonal validation

Main image

Experimental details

Immunohistochemistry analysis in human skin and skeletal muscle tissues using HPA003648 antibody. Corresponding GPR143 RNA-seq data are presented for the same tissues.

Sample type

HUMAN

Orthogonal method

Orthogonal validation of protein expression using IHC by comparison to RNA-seq data of corresponding target in high and low expression tissues.

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Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of human skin shows moderate cytoplasmic positivity in melanocytes.

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of human skin shows moderate to strong cytoplasmic positivity in melanocytes.

Sample type

HUMAN

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of human skeletal muscle shows no positivity in striated muscle fibers as expected.

Sample type

HUMAN