PAB28476

antibody from Abnova Corporation

Targeting: ZNF75A FLJ31529

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PAB28476

Provider

Abnova Corporation

Product name

ZNF75A polyclonal antibody

Antibody type

Polyclonal

Description

Rabbit polyclonal antibody raised against recombinant ZNF75A

Antigen sequence

QEEWELLDPTQKALYNDVMQENYETVISLALFVLP
KPKVISCLEQGEEPWVQVSPEFKDSAGKSPTGLKL
KNDTENHQPVSLSDLEIQASAGVISKKAKVKVPQK
TAGKENHFDMHRVGKWHQDFPVKKRKKLSTWKQEL
LKLM


Isotype

IgG

Storage

Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Western blot analysis of Lane 1: RT-4, Lane 2: U-251 MG, Lane 3: A-431, Lane 4: Liver, Lane 5: Tonsil with ZNF75A polyclonal antibody at 1:100-1:250 dilution.

Immunocytochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunofluorescent staining of human cell line A-431 with ZNF75A polyclonal antibody (Cat # PAB28476) at 1-4 ug/ml shows positivity in nucleoli & nucleus but not nucleoli.

Validation comment

Immunofluorescence

Immunohistochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunohistochemical staining of human kidney with ZNF75A polyclonal antibody (Cat # PAB28476) shows moderate cytoplasmic and nuclear positivity in cells in tubules at 1:50-1:200 dilution.

Validation comment

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)