MA5-34795

antibody from Invitrogen Antibodies

Targeting: CHAF1B CAF-1, CAF1, CAF1A, CAF1P60, MPHOSPH7, MPP7

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Recommended by provider)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

MA5-34795

Provider

Invitrogen Antibodies

Product name

CAF1 p60 Recombinant Rabbit Monoclonal Antibody (JG92-30)

Antibody type

Monoclonal

Antigen

Recombinant full-length protein

Description

Positive Control: SiHa, K562, A431, 293T, human tonsil tissue, human appendix tissue, human colon tissue.

Reactivity

Human

Host

Rabbit

Isotype

IgG

Antibody clone number

JG92-30

Vial size

100 µL

Concentration

1 mg/mL

Storage

-20° C, Avoid Freeze/Thaw Cycles, store in dark

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of p60 CAF1 in Positive control: Lane 1: SiHa, Lane 2: K562, Lane 3: A431. Samples were incubated with p60 CAF1 monoclonal antibody (Product # MA5-34795), at a dilution of 1:1000.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of p60 CAF1 in SiHa cells (green). Samples were fixed in paraformaldehyde and permeabilised with 0.25% Triton X100/PBS, incubated with p60 CAF1 monoclonal antibody (Product # MA5-34795), followed by DAPI (blue).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of p60 CAF1 in A431 cells (green). Samples were fixed in paraformaldehyde and permeabilised with 0.25% Triton X100/PBS, incubated with p60 CAF1 monoclonal antibody (Product # MA5-34795), followed by DAPI (blue).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of p60 CAF1 in 293T cells (green). Samples were fixed in paraformaldehyde and permeabilised with 0.25% Triton X100/PBS, incubated with p60 CAF1 monoclonal antibody (Product # MA5-34795), followed by DAPI (blue).

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of p60 CAF1 in paraffin-embedded human tonsil tissue. Samples were incubated with p60 CAF1 monoclonal antibody (Product # MA5-34795), and followed by hematoxylin.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of p60 CAF1 in paraffin-embedded human colon tissue. Samples were incubated with p60 CAF1 monoclonal antibody (Product # MA5-34795), and followed by hematoxylin.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of p60 CAF1 in paraffin-embedded human appendix tissue. Samples were incubated with p60 CAF1 monoclonal antibody (Product # MA5-34795), and followed by hematoxylin.

Flow cytometry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Flow cytometry of p60 CAF1 in K562 cells (purple) compared with an unlabelled control (cells without incubation with primary antibody; yellow). Samples were incubated with p60 CAF1 monoclonal antibody (Product # MA5-34795) at a dilution of 1:100, followed by Alexa Fluor 488-conjugated goat anti-rabbit IgG.