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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [2]
- Flow cytometry [1]
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Validation data
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- Product number
- TA504422 - Provider product page
- Provider
- OriGene
- Proper citation
- OriGene Cat#TA504422, RRID:AB_11124028
- Product name
- HLCS mouse monoclonal antibody, clone OTI1E4 (formerly 1E4)
- Antibody type
- Monoclonal
- Description
- HLCS mouse monoclonal antibody, clone OTI1E4 (formerly 1E4)
- Host
- Mouse
- Conjugate
- Unconjugated
- Epitope
- HLCS
- Isotype
- IgG
- Antibody clone number
- OTI1E4
- Vial size
- 100 µl
- Concentration
- 1 mg/ml
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Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY HLCS (RC209197, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-HLCS.
- Validation comment
- WB
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-HLCS mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA504422)
- Validation comment
- IHC
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-HLCS mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA504422)
- Validation comment
- IHC
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- HEK293T cells transfected with either RC209197 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-HLCS antibody(TA504422), and then analyzed by flow cytometry.
- Validation comment
- FC
