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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunohistochemistry [1]
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- Product number
- PA5-92392 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Plasma Kallikrein Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- Immunogen sequence: GCLTQLYENA FFRGGDVASM YTPNAQYCQM RCTFHPRCLL FSFLPASSIN DMEKRFGCFL KDSVTGTLPK VHRTGAVSGH SLKQCGHQIS ACHRDIYKGV DMRGVNFNVS KVSSVEECQK RCTSNIRCQF FSYATQTFHK AEYRNNCLLK YSPGGTPTAI KVLSNVESGF SLKPCALSEI GCHMNIFQHL AFSDVDVARV LTPDAFVCRT ICTYHPNCLF FTFYTNVWKI ESQRNVCLLK TSESGTPSSS TPQENTISGY SLLTCKRTLP EPCHSKIYPG V; Positive Samples: Mouse liver, Mouse intestine, Mouse brain
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 2.15 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of extracts of various cell lines, using KLKB1 Polyclonal antibody (Product # PA5-92392) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Exposure time: 30s.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of Plasma Kallikrein in various cell line extracts. 25 µg of protein was loaded in each lane. Separated proteins were transferred to membrane and probed using a Plasma Kallikrein antibody (Product # PA5-92392) diluted 1:1,000 after blocking with 3% nonfat drt milk in TBST. The blot was then probed with HRP Goat Anti-Rabbit IgG (H+L) diluted 1:10,000. ECL was used for detection.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of Plasma Kallikrein in paraffin-embedded human placenta using Plasma Kallikrein Polyclonal Antibody (Product # PA5-92392) at a dilution of 1:100.
