Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Flow cytometry [1]
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Validation data
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- Product number
- IC5875N-100UG - Provider product page
- Provider
- R&D Systems
- Product name
- Human TLR7 Alexa Fluor® 700-conjugated Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from hybridoma culture supernatant. Detects human TLR7 in direct ELISAs.
- Reactivity
- Human
- Host
- Mouse
- Conjugate
- Near infrared dye
- Antigen sequence
Q9NYK1
- Isotype
- IgG
- Antibody clone number
- 533707
- Vial size
- 100 ug
- Concentration
- 0.2 mg/ml
- Storage
- Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied.
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Supportive validation
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- R&D Systems (provider)
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- Experimental details
- Detection of TLR7 in Ramos Human Cell Line by Flow Cytometry. Ramos human Burkitt's lymphoma cell line was stained with Mouse Anti-Human TLR7 Alexa Fluor® 700-conjugated Monoclonal Antibody (Catalog # IC5875N, filled histogram) or isotype control antibody (Catalog # IC003N, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer (Catalog # FC005). View our protocol for Staining Intracellular Molecules.