50-7359-41

antibody from Invitrogen Antibodies

Targeting: IL12A CLMF, IL-12A, NFSK, NKSF1, p35

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

50-7359-41

Provider

Invitrogen Antibodies

Product name

IL-12 p35 Monoclonal Antibody (SNKY35), eFluor™ 660, eBioscience™

Antibody type

Monoclonal

Antigen

Other

Description

Description: This SNKY35 monoclonal antibody reacts with human p35, a component of two of the four members of the interleukin (IL)-12 family of cytokines. The IL-12 family members are heterodimeric cytokines composed of an alpha-chain, such as p35, and a beta-chain, such as p40. The alpha-chain proteins are thought to be the limiting factors for secretion of IL-12 family member cytokines, as expression of the alpha-chain is more restricted than that of the beta-chain proteins, and the two subunits may need to be co-expressed in order for cytokine secretion to occur. IL-12 family members are secreted primarily by antigen-presenting cells in response to various TLR agonists and other inflammatory stimuli. The exact types of stimuli present and kinetics of stimulation determine the balance of IL-12 family member production.

Antibody clone number

SNKY35

Concentration

5 µL/Test

References

Submitted references

Single-cell transcriptomic analysis reveals disparate effector differentiation pathways in human T(reg) compartment.

Luo Y, Xu C, Wang B, Niu Q, Su X, Bai Y, Zhu S, Zhao C, Sun Y, Wang J, Liu M, Sun X, Song G, Cui H, Chen X, Huang H, Wang H, Han M, Jiang E, Shi L, Feng X
Nature communications 2021 Jun 23;12(1):3913

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Prostaglandin E2 inhibits IL-23 and IL-12 production by human monocytes through down-regulation of their common p40 subunit.

Kalim KW, Groettrup M
Molecular immunology 2013 Mar;53(3):274-82

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IL-35-mediated induction of a potent regulatory T cell population.

Collison LW, Chaturvedi V, Henderson AL, Giacomin PR, Guy C, Bankoti J, Finkelstein D, Forbes K, Workman CJ, Brown SA, Rehg JE, Jones ML, Ni HT, Artis D, Turk MJ, Vignali DA
Nature immunology 2010 Dec;11(12):1093-101

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How microorganisms tip the balance between interleukin-12 family members.

Goriely S, Neurath MF, Goldman M
Nature reviews. Immunology 2008 Jan;8(1):81-6

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Interleukin-35: odd one out or part of the family?

Collison LW, Vignali DA
Immunological reviews 2008 Dec;226:248-62

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Two-sided roles of IL-27: induction of Th1 differentiation on naive CD4+ T cells versus suppression of proinflammatory cytokine production including IL-23-induced IL-17 on activated CD4+ T cells partially through STAT3-dependent mechanism.

Yoshimura T, Takeda A, Hamano S, Miyazaki Y, Kinjyo I, Ishibashi T, Yoshimura A, Yoshida H
Journal of immunology (Baltimore, Md. : 1950) 2006 Oct 15;177(8):5377-85

Flow cytometry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Normal human peripheral blood cells stimulated with Human IFN gamma Recombinant Protein (Product # 14-8319-80), LPS, and the Imidazoquinoline, R848, in the presence of Protein Transport Inhibitor Cocktail (Product # 00-4980-03) and stained intracellularly with Anti-Human IL-12/23 p40 PE (Product # 12-7235-42) and Mouse IgG1 K Isotype Control (Product # 50-4714-82) (left) or Anti-Human IL-12 p35 eFluor® 660 (right). Cells in the monocyte gate were used for analysis.