Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [1]
- Flow cytometry [2]
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Validation data
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- Product number
- AF1148 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Goat Polyclonal Coagulation Factor II/Thrombin Antibody
- Antibody type
- Polyclonal
- Description
- Antigen Affinity-purified. Detects human Coagulation Factor II/Thrombin in direct ELISAs and Western blots. In direct ELISAs, approximately 10% cross-reactivity with recombinant human (rh) PROC is observed, and less than 1% cross-reactivity with rhFactor X, recombinant mouse (rm) Factor X and rmPROC is observed.
- Reactivity
- Human
- Host
- Goat
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.
Submitted references Protease-activated receptor 2 blocking peptide counteracts endotoxin-induced inflammation and coagulation and ameliorates renal fibrin deposition in a rat model of acute renal failure.
Jesmin S, Gando S, Zaedi S, Prodhan SH, Sawamura A, Miyauchi T, Hiroe M, Yamaguchi N
Shock (Augusta, Ga.) 2009 Dec;32(6):626-32
Shock (Augusta, Ga.) 2009 Dec;32(6):626-32
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Detection of Human Coagulation Factor II/Thrombin by Simple WesternTM. Simple Western lane view shows lysates of human liver tissue, loaded at 0.2 mg/mL. A specific band was detected for Coagulation Factor II/Thrombin at approximately 95 kDa (as indicated) using 10 µg/mL of Goat Anti-Human Coagulation Factor II/Thrombin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1148) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Detection of Coagulation Factor II/Thrombin in Human Blood Monocytes by Flow Cytometry. Human peripheral blood monocytes were stained with Goat Anti-Human Coagulation Factor II/Thrombin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1148, filled histogram) or isotype control antibody (Catalog # AB-108-C, open histogram), followed by Phycoerythrin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0107).
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Detection of Human Factor II/Thrombin in HepG2 Human Cell Line by Flow Cytometry.