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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [2]
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- Product number
- MAB1938 - Provider product page
- Provider
- R&D Systems
- Product name
- Human alpha 2-Macroglobulin Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from hybridoma culture supernatant. Detects human alpha 2-Macroglobulin in direct ELISAs and Western blots. In direct ELISAs, this antibody does not cross-react with rhC5a or rmC5a.
- Reactivity
- Human
- Host
- Mouse
- Conjugate
- Unconjugated
- Isotype
- IgG
- Antibody clone number
- 257316
- Vial size
- 500 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references Maggot chymotrypsin I from Lucilia sericata is resistant to endogenous wound protease inhibitors.
Telford G, Brown AP, Kind A, English JS, Pritchard DI
The British journal of dermatology 2011 Jan;164(1):192-6
The British journal of dermatology 2011 Jan;164(1):192-6
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of Human alpha 2-Macroglobulin by Western Blot. Western blot shows human serum. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human alpha 2-Macroglobulin Monoclonal Antibody (Catalog # MAB1938) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for alpha 2-Macroglobulin at approximately 180 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of Human alpha 2-Macroglobulin by Simple WesternTM. Simple Western lane view shows human serum, loaded at 0.2 mg/mL. A specific band was detected for alpha 2-Macroglobulin at approximately 178 kDa (as indicated) using 10 µg/mL of Mouse Anti-Human alpha 2-Macroglobulin Monoclonal Antibody (Catalog # MAB1938) . This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.
