200-301-388

antibody from Rockland Immunochemicals, Inc.

Targeting: TG AITD3, TGN

Western blot (Supportive data in Antibodypedia)

Antibody Data

Product number

200-301-388

Provider

Rockland Immunochemicals, Inc.

Proper citation

Rockland Cat#200-301-388, RRID:AB_2201636

Product name

Anti-Thyroglobulin (MOUSE) Monoclonal Antibody - 200-301-388

Antibody type

Monoclonal

Vial size

100 µl

Western blot

Supportive validation

Submitted by

Rockland Immunochemicals, Inc. (provider)

Main image

Experimental details

Western blot using ROCKLAND Immunochemical's Mouse Mab-anti-Thyroglobulin antibody. Separation was achieved under reducing conditions using a pre-cast 5% Tris-HCl gel from Bio-Rad Laboratories. This antibody recognizes a single 330 kDa band corresponding to human thyroglobulin (left lane 3 ?g, right lane 3.0 ?g) as confirmed by the position of molecular weight markers (not shown). A 1:400 dilution of Mab anti-Thyroglobulin was used for 2h followed by detection using a 1:5,000 dilution of IRDyeTM800 conjugated Goat-a-Mouse IgG [H&L] (610-132-121) and visualization using the Odyssey® Infrared Imaging System developed by LI-COR. Other detection systems will yield similar results. IRDye is a trademark of LI-COR, Inc.

Validation comment

Western Blot