R1601P

antibody from Acris Antibodies GmbH

Targeting: TG AITD3, TGN

Western blot (Supportive data in Antibodypedia)

ELISA (Recommended by provider)

Immunoprecipitation (Recommended by provider)

Immunohistochemistry (Recommended by provider)

Antibody Data

Product number

R1601P

Provider

Acris Antibodies GmbH

Proper citation

Acris Antibodies GmbH Cat#R1601P, RRID:AB_1007949

Product name

anti Thyroglobulin (Tg)

Antibody type

Monoclonal

Antigen

This protein A purified monoclonal antibody was produced by repeated immunizations with human thyroglobulin protein. Produced by the fusion between BALB/c mouse splenocytes and mouse myeloma SP2/0 cells after immunization with thyroglobulin from human thyroid gland using conventional hybridoma technology.

Reactivity

Human, Mouse, Rat, Canine

Host

Mouse

Isotype

IgG

Antibody clone number

2H11

Vial size

0.1 mg

Concentration

1.6 mg/ml (by UV absorbance at 280 nm)

Western blot

Supportive validation

Submitted by

Acris Antibodies GmbH (provider)

Main image

Experimental details

Figure 1. Western blot using Immunochemical's Mouse anti-Thyroglobulin antibody (R1601P). Separation was achieved under reducing conditions using a pre-cast 5% Tris-HCl gel from Bio-Rad Laboratories. This antibody recognizes a single 330 kDa band corresponding to human thyroglobulin (left lane 3 µg, right lane 3.0 µg) as confirmed by the position of molecular weight markers (not shown). A 1/400 dilution of Mab anti-Thyroglobulin was used for 2h followed by detection using a 1/5,000 dilution of IRDyeTM800 conjugated Goat-a-Mouse IgG [H&L] and visualization using the Odyssey ¨ Infrared Imaging System developed by LI-COR. Other detection systems will yield similar results. IRDye is a trademark of LI-COR, Inc.