11486-2-AP

antibody from Proteintech Group

Targeting: APOL1 APOL

Western blot (Supportive data in Antibodypedia)

ELISA (Data presented on provider website)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Data presented on provider website)

Antibody Data

Product number

11486-2-AP

Provider

Proteintech Group

Proper citation

Proteintech Cat#11486-2-AP, RRID:AB_2058396

Product name

APOL1 antibody

Antibody type

Polyclonal

Description

KD/KO validated APOL1 antibody (Cat. #11486-2-AP) is a rabbit polyclonal antibody that shows reactivity with human and has been validated for the following applications: FC, IF, IHC, IP, WB, ELISA.

Reactivity

Human

Host

Rabbit

Conjugate

Unconjugated

Isotype

IgG

Vial size

20ul, 150ul

References

Submitted references

Interplay of Trypanosome Lytic Factor and innate immune cells in the resolution of cutaneous Leishmania infection.

Pant J, Samanovic M, Nelson MT, Keceli MK, Verdi J, Beverley SM, Raper J
PLoS pathogens 2021 Sep;17(9):e1008768

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Structures of the ApoL1 and ApoL2 N-terminal domains reveal a non-classical four-helix bundle motif.

Ultsch M, Holliday MJ, Gerhardy S, Moran P, Scales SJ, Gupta N, Oltrabella F, Chiu C, Fairbrother W, Eigenbrot C, Kirchhofer D
Communications biology 2021 Jul 27;4(1):916

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Genome-Wide Identification of Autophagy Prognostic Signature in Pancreatic Cancer.

Yu J, Lang Q, Zhong C, Wang S, Tian Y
Dose-response : a publication of International Hormesis Society 2021 Apr-Jun;19(2):15593258211023260

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APOL1 risk variants affect podocyte lipid homeostasis and energy production in focal segmental glomerulosclerosis.

Ge M, Molina J, Ducasa GM, Mallela SK, Varona Santos J, Mitrofanova A, Kim JJ, Liu X, Sloan A, Mendez AJ, Banerjee S, Liu S, Szeto HH, Shin MK, Hoek M, Kopp JB, Fontanesi F, Merscher S, Fornoni A
Human molecular genetics 2021 Apr 26;30(3-4):182-197

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All You Ever Wanted to Know About APOL1 and TLFs and Did Not Dare Ask.

Verdi J, Schaub C, Thomson R, Raper J
Methods in molecular biology (Clifton, N.J.) 2020;2116:463-483

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Domain-Specific Antibodies Reveal Differences in the Membrane Topologies of Apolipoprotein L1 in Serum and Podocytes.

Gupta N, Wang X, Wen X, Moran P, Paluch M, Hass PE, Heidersbach A, Haley B, Kirchhofer D, Brezski RJ, Peterson AS, Scales SJ
Journal of the American Society of Nephrology : JASN 2020 Sep;31(9):2065-2082

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Apolipoprotein L1-Specific Antibodies Detect Endogenous APOL1 inside the Endoplasmic Reticulum and on the Plasma Membrane of Podocytes.

Scales SJ, Gupta N, De Mazière AM, Posthuma G, Chiu CP, Pierce AA, Hötzel K, Tao J, Foreman O, Koukos G, Oltrabella F, Klumperman J, Lin W, Peterson AS
Journal of the American Society of Nephrology : JASN 2020 Sep;31(9):2044-2064

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Apolipoprotein L-1 renal risk variants form active channels at the plasma membrane driving cytotoxicity.

Giovinazzo JA, Thomson RP, Khalizova N, Zager PJ, Malani N, Rodriguez-Boulan E, Raper J, Schreiner R
eLife 2020 May 19;9

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Vacuolar ATPase depletion contributes to dysregulation of endocytosis in bloodstream forms of Trypanosoma brucei.

Xu ZS, Li FJ, Hide G, Lun ZR, Lai DH
Parasites & vectors 2020 Apr 25;13(1):214

---

MiR193a Modulation and Podocyte Phenotype.

Jha A, Saha S, Ayasolla K, Vashistha H, Malhotra A, Skorecki K, Singhal PC
Cells 2020 Apr 17;9(4)

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Antisense oligonucleotide treatment ameliorates IFN-γ-induced proteinuria in APOL1-transgenic mice.

Aghajan M, Booten SL, Althage M, Hart CE, Ericsson A, Maxvall I, Ochaba J, Menschik-Lundin A, Hartleib J, Kuntz S, Gattis D, Ahlström C, Watt AT, Engelhardt JA, Monia BP, Magnone MC, Guo S
JCI insight 2019 Jun 20;4(12)

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Basal expression of interferon regulatory factor 1 drives intrinsic hepatocyte resistance to multiple RNA viruses.

Yamane D, Feng H, Rivera-Serrano EE, Selitsky SR, Hirai-Yuki A, Das A, McKnight KL, Misumi I, Hensley L, Lovell W, González-López O, Suzuki R, Matsuda M, Nakanishi H, Ohto-Nakanishi T, Hishiki T, Wauthier E, Oikawa T, Morita K, Reid LM, Sethupathy P, Kohara M, Whitmire JK, Lemon SM
Nature microbiology 2019 Jul;4(7):1096-1104

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The effect of normal human serum on the mouse trypanosome Trypanosoma musculi in vitro and in vivo.

Zhang X, Hong XK, Li SJ, Lai DH, Hide G, Lun ZR, Wen YZ
Experimental parasitology 2018 Jan;184:115-120

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A new panel of pancreatic cancer biomarkers discovered using a mass spectrometry-based pipeline.

Liu X, Zheng W, Wang W, Shen H, Liu L, Lou W, Wang X, Yang P
British journal of cancer 2017 Dec 5;117(12):1846-1854

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Knockdown of ApoL1 in Zebrafish Larvae Affects the Glomerular Filtration Barrier and the Expression of Nephrin.

Kotb AM, Simon O, Blumenthal A, Vogelgesang S, Dombrowski F, Amann K, Zimmermann U, Endlich K, Endlich N
PloS one 2016;11(5):e0153768

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BH3 domain-independent apolipoprotein L1 toxicity rescued by BCL2 prosurvival proteins.

Heneghan JF, Vandorpe DH, Shmukler BE, Giovinazzo JA, Raper J, Friedman DJ, Pollak MR, Alper SL
American journal of physiology. Cell physiology 2015 Sep 1;309(5):C332-47

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Evolution of the primate trypanolytic factor APOL1.

Thomson R, Genovese G, Canon C, Kovacsics D, Higgins MK, Carrington M, Winkler CA, Kopp J, Rotimi C, Adeyemo A, Doumatey A, Ayodo G, Alper SL, Pollak MR, Friedman DJ, Raper J
Proceedings of the National Academy of Sciences of the United States of America 2014 May 20;111(20):E2130-9

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Comparative proteomic profiling of plasma very-low-density and low-density lipoproteins.

Sun HY, Chen SF, Lai MD, Chang TT, Chen TL, Li PY, Shieh DB, Young KC
Clinica chimica acta; international journal of clinical chemistry 2010 Mar;411(5-6):336-44

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Hydrodynamic gene delivery of baboon trypanosome lytic factor eliminates both animal and human-infective African trypanosomes.

Thomson R, Molina-Portela P, Mott H, Carrington M, Raper J
Proceedings of the National Academy of Sciences of the United States of America 2009 Nov 17;106(46):19509-14

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Distinct roles of apolipoprotein components within the trypanosome lytic factor complex revealed in a novel transgenic mouse model.

Molina-Portela MP, Samanovic M, Raper J
The Journal of experimental medicine 2008 Aug 4;205(8):1721-8

Western blot

Supportive validation

Submitted by

Proteintech Group (provider)

Main image

Experimental details

human blood tissue were subjected to SDS PAGE followed by western blot with 11486-2-AP(APOL1 antibody) at dilution of 1:1000

Sample type

tissue

Immunocytochemistry

Supportive validation

Submitted by

Proteintech Group (provider)

Main image

Experimental details

Immunofluorescent analysis of HepG2 cells, using APOL1 antibody 11486-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

Sample type

cell line

Immunoprecipitation

Supportive validation

Submitted by

Proteintech Group (provider)

Main image

Experimental details

IP Result of anti-APOL1 (IP:11486-2-AP, 3ug; Detection:11486-2-AP 1:500) with HepG2 cells lysate 6000ug.

Sample type

cell line

Immunohistochemistry

Supportive validation

Submitted by

Proteintech Group (provider)

Main image

Experimental details

Immunohistochemical of paraffin-embedded human kidney using 11486-2-AP(APOL1 antibody) at dilution of 1:50 (under 10x lens)

Sample type

tissue

Supportive validation

Submitted by

Proteintech Group (provider)

Main image

Experimental details

Immunohistochemical of paraffin-embedded human kidney using 11486-2-AP(APOL1 antibody) at dilution of 1:50 (under 40x lens)

Sample type

tissue