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LearnH00000259-M01
antibody from Abnova Corporation
Targeting: AMBP
EDC1, HCP, HI30, IATIL, ITI, ITIL, ITILC, UTI
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [2]
- ELISA [1]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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Validation data
Reference
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- Product number
- H00000259-M01 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#H00000259-M01, RRID:AB_425300
- Product name
- AMBP monoclonal antibody (M01), clone 3F1
- Antibody type
- Monoclonal
- Description
- Mouse monoclonal antibody raised against a full length recombinant AMBP.
- Antigen sequence
AGPVPTPPDNIQVQGNFNISRIYGKWYNLAIGSTC
PWLKKIMDRMTVSTLVLGEGATEAEISMTSTRWRK
GVCEETSGAYEKTDTDGKFLYHKSKWNITMESYVV
HTNYDEYAIFLTKKFSRHHGPTITAKLYGRAPQLR
ETLLQDFRVVAQGVGIPEDSIFTMADRGECVPGEQ
EPEPILIPRVRRAVLPQEEEGSGGGQLVTEVTKKE
DSCQLGYSAGPCMGMTSRYFYNGTSMACETFQYGG
CMGNGNNFVTEKECLQTCRTVAACNLPIVRGPCRA
FIQLWAFDAVKGKCVLFPYGGCQGNGNKFYSEKEC
REYCGVPGDGDEELLRFSN- Isotype
- IgG
- Antibody clone number
- 3F1
- Storage
- Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
Submitted references Differential proteomics analysis of amniotic fluid in pregnancies of increased nuchal translucency with normal karyotype.
Cheng PJ, Wang TH, Huang SY, Kao CC, Lu JH, Hsiao CH, Shaw SW
Prenatal diagnosis 2011 Mar;31(3):274-81
Prenatal diagnosis 2011 Mar;31(3):274-81
No comments: Submit comment
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- AMBP monoclonal antibody (M01), clone 3F1 Western Blot analysis of AMBP expression in A-549 ( Cat # L025V1 ).
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Western Blot analysis of AMBP expression in transfected 293T cell line by AMBP monoclonal antibody (M01), clone 3F1.Lane 1: AMBP transfected lysate(39 KDa).Lane 2: Non-transfected lysate.
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Detection limit for recombinant GST tagged AMBP is approximately 1ng/ml as a capture antibody.
- Validation comment
- Sandwich ELISA (Recombinant protein)
- Protocol
- Protocol
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Immunofluorescence of monoclonal antibody to AMBP on HeLa cell. [antibody concentration 10 ug/ml]
- Validation comment
- Immunofluorescence
- Protocol
- Protocol
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Immunoperoxidase of monoclonal antibody to AMBP on formalin-fixed paraffin-embedded human liver. [antibody concentration 1 ug/ml]
- Validation comment
- Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
- Protocol
- Protocol
