PA5-80336

antibody from Invitrogen Antibodies

Targeting: AMBP EDC1, HCP, HI30, IATIL, ITI, ITIL, ITILC, UTI

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-80336

Provider

Invitrogen Antibodies

Product name

alpha-1 Microglobulin Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant full-length protein

Description

This product is preservative free. It is recommended to add sodium azide to avoid contamination (final concentration 0.05%-0.1%).

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of alpha-1 Microglobulin in Lane A: A549 Whole Cell Lysate (30 µg). Samples were probed using a alpha-1 Microglobulin Polyclonal Antibody (Product # PA5-80336) at a 1:500 dilution, followed by a Goat Anti-Rabbit IgG (H+L), Dylight 800 Secondary Antibody at a 1:10000 dilution. Western blot was performed under reducing conditions. Predicted band size:39 kDa. Observed band size:34 kDa.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot using alpha-1 Microglobulin Polyclonal Antibody (Product # PA5-80336) at 1:500 dilution. Lane A: A549 Whole Cell Lysate. Lysates/proteins at 30 μg per lane. Secondary Goat Anti-Rabbit IgG H&L (DyLight™ 800) at 1:10,000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 39 kDa. Observed band size: 34 kDa.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of human alpha-1-microglobulin in human liver with alpha-1 Microglobulin Polyclonal Antibody (Product # PA5-80336, 1:10,000, formalin-fixed paraffin embedded sections).

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

alpha-1 Microglobulin Immunoprecipitation using: Lane A: 0.5 mg A549 Whole Cell Lysate 0.5 µL with alpha-1 Microglobulin Polyclonal Antibody (Product # PA5-80336) and 15 µL of 50 % Protein G agarose. Primary antibody: alpha-1 Microglobulin Polyclonal Antibody, at 1:500 dilution. Secondary antibody: Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5,000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 34 kDa. Observed band size: 34 kDa.