PA5-27314

antibody from Invitrogen Antibodies

Targeting: AMBP EDC1, HCP, HI30, IATIL, ITI, ITIL, ITILC, UTI

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-27314

Provider

Invitrogen Antibodies

Product name

alpha-1 Microglobulin Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant protein fragment

Description

Recommended positive controls: 293T, A431, human plasma, HepG2 conditioned medium.

Concentration

1 mg/mL

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot analysis of alpha-1 Microglobulin was performed by separating HepG2 whole cell extract and conditioned medium (30 µg) by 10% SDS-PAGE. The membrane was blotted with an alpha-1 Microglobulin Polyclonal Antibody (Product # PA5-27314) diluted at 1:100000.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot analysis of alpha-1 Microglobulin was performed by separating 30 µg of human tissue extract by 10% SDS-PAGE. Proteins were transferred to a membrane and probed with a alpha-1 Microglobulin Polyclonal Antibody (Product # PA5-27314) at a dilution of 1:5000.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot using alpha-1 Microglobulin Polyclonal Antibody (Product # PA5-27314). Sample (30 µg of whole cell lysate). Lane A: 293T. Lane B: A431. 10% SDS PAGE. alpha-1 Microglobulin Polyclonal Antibody (Product # PA5-27314) diluted at 1:5,000.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

alpha-1-Microglobulin antibody detects alpha-1-Microglobulin protein at cytoplasm by immunofluorescent analysis. Sample: A431 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: alpha-1-Microglobulin protein stained by alpha-1-Microglobulin antibody (Product # PA5-27314) diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar = 10 μm.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical analysis of paraffin-embedded human hepatoma, using alpha-1-Microglobulin (Product # PA5-27314) antibody at 1:500 dilution. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.