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LearnPA5-27314
antibody from Invitrogen Antibodies
Targeting: AMBP
EDC1, HCP, HI30, IATIL, ITI, ITIL, ITILC, UTI
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [3]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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- Product number
- PA5-27314 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- alpha-1 Microglobulin Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: 293T, A431, human plasma, HepG2 conditioned medium. Predicted reactivity: Cat (81%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of alpha-1 Microglobulin was performed by separating HepG2 whole cell extract and conditioned medium (30 µg) by 10% SDS-PAGE. The membrane was blotted with an alpha-1 Microglobulin Polyclonal Antibody (Product # PA5-27314) diluted at 1:100000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of alpha-1 Microglobulin was performed by separating 30 µg of human tissue extract by 10% SDS-PAGE. Proteins were transferred to a membrane and probed with a alpha-1 Microglobulin Polyclonal Antibody (Product # PA5-27314) at a dilution of 1:5000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using alpha-1 Microglobulin Polyclonal Antibody (Product # PA5-27314). Sample (30 µg of whole cell lysate). Lane A: 293T. Lane B: A431. 10% SDS PAGE. alpha-1 Microglobulin Polyclonal Antibody (Product # PA5-27314) diluted at 1:5,000.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- alpha-1-Microglobulin antibody detects alpha-1-Microglobulin protein at cytoplasm by immunofluorescent analysis. Sample: A431 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: alpha-1-Microglobulin protein stained by alpha-1-Microglobulin antibody (Product # PA5-27314) diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar = 10 μm.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded human hepatoma, using alpha-1-Microglobulin (Product # PA5-27314) antibody at 1:500 dilution. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
