15274-1-AP

antibody from Proteintech Group

Targeting: SPARC BM-40, ON, ONT

Western blot (Supportive data in Antibodypedia)

ELISA (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

15274-1-AP

Provider

Proteintech Group

Proper citation

Proteintech Cat#15274-1-AP, RRID:AB_2194961

Product name

SPARC antibody

Antibody type

Polyclonal

Description

SPARC antibody (Cat. #15274-1-AP) is a rabbit polyclonal antibody that shows reactivity with human, mouse, rat, pig and has been validated for the following applications: IHC, WB,ELISA.

Reactivity

Human, Mouse, Rat, Porcine

Host

Rabbit

Conjugate

Unconjugated

Isotype

IgG

Vial size

20ul, 150ul

References

Submitted references

SEC23A Inhibit Melanoma Metastatic through Secretory PF4 Cooperation with SPARC to Inhibit MAPK Signaling Pathway.

Zeng B, Sun Z, Zhao Q, Liu D, Chen H, Li X, Xing HR, Wang J
International journal of biological sciences 2021;17(12):3000-3012

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A 9-kDa matricellular SPARC fragment released by cathepsin D exhibits pro-tumor activity in the triple-negative breast cancer microenvironment.

Alcaraz LB, Mallavialle A, David T, Derocq D, Delolme F, Dieryckx C, Mollevi C, Boissière-Michot F, Simony-Lafontaine J, Du Manoir S, Huesgen PF, Overall CM, Tartare-Deckert S, Jacot W, Chardès T, Guiu S, Roger P, Reinheckel T, Moali C, Liaudet-Coopman E
Theranostics 2021;11(13):6173-6192

---

SARS-CoV-2 Disrupts Proximal Elements in the JAK-STAT Pathway.

Chen DY, Khan N, Close BJ, Goel RK, Blum B, Tavares AH, Kenney D, Conway HL, Ewoldt JK, Chitalia VC, Crossland NA, Chen CS, Kotton DN, Baker SC, Fuchs SY, Connor JH, Douam F, Emili A, Saeed M
Journal of virology 2021 Sep 9;95(19):e0086221

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SPARC knockdown attenuated TGF-β1-induced fibrotic effects through Smad2/3 pathways in human pterygium fibroblasts.

Fan J, Zhang X, Jiang Y, Chen L, Sheng M, Chen Y
Archives of biochemistry and biophysics 2021 Nov 30;713:109049

---

Evidence for Delivery of Abraxane via a Denatured-Albumin Transport System.

Hama M, Ishima Y, Chuang VTG, Ando H, Shimizu T, Ishida T
ACS applied materials & interfaces 2021 May 5;13(17):19736-19744

---

Identification of Overexpressed Genes in Malignant Pleural Mesothelioma.

Morani F, Bisceglia L, Rosini G, Mutti L, Melaiu O, Landi S, Gemignani F
International journal of molecular sciences 2021 Mar 8;22(5)

---

The "Matrisome" reveals the characterization of skin keloid microenvironment.

Zhang S, Liu B, Wang W, Lv L, Gao D, Chai M, Li M, Wu Z, Zhu Y, Ma J, Leng L
FASEB journal : official publication of the Federation of American Societies for Experimental Biology 2021 Apr;35(4):e21237

---

MicroRNA‑592 promotes cell proliferation, migration and invasion in colorectal cancer by directly targeting SPARC.

Pan Z, Xie R, Song W, Gao C
Molecular medicine reports 2021 Apr;23(4)

---

SARS-CoV-2 desensitizes host cells to interferon through inhibition of the JAK-STAT pathway.

Chen DY, Khan N, Close BJ, Goel RK, Blum B, Tavares AH, Kenney D, Conway HL, Ewoldt JK, Kapell S, Chitalia VC, Crossland NA, Chen CS, Kotton DN, Baker SC, Connor JH, Douam F, Emili A, Saeed M
bioRxiv : the preprint server for biology 2020 Oct 28;

---

Induction of SPARC on Oxidative Stress, Inflammatory Phenotype Transformation, and Apoptosis of Human Brain Smooth Muscle Cells Via TGF-β1-NOX4 Pathway.

Tan X, Li T, Zhu S, Zhong W, Li F, Wang Y
Journal of molecular neuroscience : MN 2020 Nov;70(11):1728-1741

---

Effects of transforming growth factor-β1 on plasminogen activation in stem cells from the apical papilla: role of activating receptor-like kinase 5/Smad2 and mitogen-activated protein kinase kinase (MEK)/extracellular signal-regulated kinase (ERK) signalling.

Chang MC, Chang HH, Hsieh WC, Huang WL, Lian YC, Jeng PY, Wang YL, Yeung SY, Jeng JH
International endodontic journal 2020 May;53(5):647-659

---

Antimetastatic Effect of Fucoidan-Sargassum against Liver Cancer Cell Invadopodia Formation via Targeting Integrin αVβ3 and Mediating αVβ3/Src/E2F1 Signaling.

Pan TJ, Li LX, Zhang JW, Yang ZS, Shi DM, Yang YK, Wu WZ
Journal of Cancer 2019;10(20):4777-4792

---

Olfactory ensheathing cells facilitate neurite sprouting and outgrowth by secreting high levels of hevin.

Ge L, Zhuo Y, Wu P, Liu Y, Qi L, Teng X, Duan D, Chen P, Lu M
Journal of chemical neuroanatomy 2019 Nov 26;104:101728

---

Quantitative proteomic analysis of deer antler stem cells as a model of mammalian organ regeneration.

Dong Z, Coates D, Liu Q, Sun H, Li C
Journal of proteomics 2019 Mar 20;195:98-113

---

Effects of hydrogen peroxide on biological characteristics and osteoinductivity of decellularized and demineralized bone matrices.

Qing Q, Zhang YJ, Yang JL, Ning LJ, Zhang YJ, Jiang YL, Zhang Y, Luo JC, Qin TW
Journal of biomedical materials research. Part A 2019 Jul;107(7):1476-1490

---

Myosin-X Silencing in the Trabecular Meshwork Suggests a Role for Tunneling Nanotubes in Outflow Regulation.

Sun YY, Yang YF, Keller KE
Investigative ophthalmology & visual science 2019 Feb 1;60(2):843-851

---

Transcriptome analysis of gingival tissues of enamel-renal syndrome.

Wang YP, Lin HY, Zhong WL, Simmer JP, Wang SK
Journal of periodontal research 2019 Dec;54(6):653-661

---

Priming integrin α5 promotes human dental pulp stem cells odontogenic differentiation due to extracellular matrix deposition and amplified extracellular matrix-receptor activity.

Wang H, Ning T, Song C, Luo X, Xu S, Zhang X, Deng Z, Ma D, Wu B
Journal of cellular physiology 2019 Aug;234(8):12897-12909

---

Label-free quantitative mass spectrometry analysis of differential protein expression in the developing cochlear sensory epithelium.

Darville LNF, Sokolowski BHA
Proteome science 2018;16:15

---

Proteomic Analysis of Plasma Membrane Proteins of Antler Stem Cells Using Label-Free LC⁻MS/MS.

Wang D, Ba H, Li C, Zhao Q, Li C
International journal of molecular sciences 2018 Nov 5;19(11)

---

StarD13 3'-untranslated region functions as a ceRNA for TP53INP1 in prohibiting migration and invasion of breast cancer cells by regulating miR-125b activity.

Zheng L, Li X, Chou J, Xiang C, Guo Q, Zhang Z, Guo X, Gao L, Xing Y, Xi T
European journal of cell biology 2018 Jan;97(1):23-31

---

miR-504 promotes tumour growth and metastasis in human osteosarcoma by targeting TP53INP1.

Cai Q, Zeng S, Dai X, Wu J, Ma W
Oncology reports 2017 Nov;38(5):2993-3000

---

Mapping molecular differences and extracellular matrix gene expression in segmental outflow pathways of the human ocular trabecular meshwork.

Vranka JA, Bradley JM, Yang YF, Keller KE, Acott TS
PloS one 2015;10(3):e0122483

---

Analysis of colorectal cancer glyco-secretome identifies laminin β-1 (LAMB1) as a potential serological biomarker for colorectal cancer.

Lin Q, Lim HS, Lin HL, Tan HT, Lim TK, Cheong WK, Cheah PY, Tang CL, Chow PK, Chung MC
Proteomics 2015 Nov;15(22):3905-20

---

Identification of colonic fibroblast secretomes reveals secretory factors regulating colon cancer cell proliferation.

Chen SX, Xu XE, Wang XQ, Cui SJ, Xu LL, Jiang YH, Zhang Y, Yan HB, Zhang Q, Qiao J, Yang PY, Liu F
Journal of proteomics 2014 Oct 14;110:155-71

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Expression of Secreted Protein Acidic and Rich in Cysteine in the Stroma of a Colorectal Carcinoma is Associated With Patient Prognosis.

Kim JY, Jeong D, Ahn TS, Kim HJ, Park DS, Park SY, Bae SB, Lee S, Lee SS, Lee MS, Cho HD, Baek MJ
Annals of coloproctology 2013 Jun;29(3):93-9

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nab-Paclitaxel potentiates gemcitabine activity by reducing cytidine deaminase levels in a mouse model of pancreatic cancer.

Frese KK, Neesse A, Cook N, Bapiro TE, Lolkema MP, Jodrell DI, Tuveson DA
Cancer discovery 2012 Mar;2(3):260-269

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Mining the gastric cancer secretome: identification of GRN as a potential diagnostic marker for early gastric cancer.

Loei H, Tan HT, Lim TK, Lim KH, So JB, Yeoh KG, Chung MC
Journal of proteome research 2012 Mar 2;11(3):1759-72

---

The pancreas cancer microenvironment.

Feig C, Gopinathan A, Neesse A, Chan DS, Cook N, Tuveson DA
Clinical cancer research : an official journal of the American Association for Cancer Research 2012 Aug 15;18(16):4266-76

Western blot

Supportive validation

Submitted by

Proteintech Group (provider)

Main image

Experimental details

human placenta tissue were subjected to SDS PAGE followed by western blot with 15274-1-AP(SPARC antibody) at dilution of 1:500

Sample type

tissue

Immunohistochemistry

Supportive validation

Submitted by

Proteintech Group (provider)

Main image

Experimental details

Immunohistochemical of paraffin-embedded human stomach cancer using 15274-1-AP(SPARC antibody) at dilution of 1:100 (under 10x lens)

Sample type

tissue

Supportive validation

Submitted by

Proteintech Group (provider)

Main image

Experimental details

The SPARC antibody from Proteintech is a rabbit polyclonal antibody to a recombinant protein of human SPARC. This antibody recognizes human, mouse, rat, pig antigen. The SPARC antibody has been validated for the following applications: ELISA, WB, IHC analysis.

Appendix

15274-1-AP.pdf

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