Explore
Validate
Learn
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunohistochemistry [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- R30662 - Provider product page
- Provider
- NSJ Bioreagents
- Product name
- SPARC Antibody
- Antibody type
- Polyclonal
- Antigen
- Amino acids 284-303 (DEWAGCFGIKQKDIDKDLVI-human) were used as the immunogen for this SPARC antibody.
- Description
- Antigen affinity purified antibody
- Reactivity
- Human
- Host
- Rabbit
- Conjugate
- Unconjugated
- Vial size
- 100 µg
- Concentration
- Lyophilized; resuspend with 200 ul for 0.5 mg/ml
- Storage
- After reconstitution, the SPARC antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.
No comments: Submit comment
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Western blot testing of SPARC antibody and HeLa cell lysate. The glycoprotein is routinely visualized from 35~43KD.
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Western blot testing of SPARC antibody and Lane 1: recombinant human protein 10ng; 2: 5ng; 3: 2.5ng
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- IHC-P: SPARC antibody testing of human intestinal cancer tissue
