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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [3]
- ELISA [1]
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- Product number
- H00006678-M02 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#H00006678-M02, RRID:AB_913759
- Product name
- SPARC monoclonal antibody (M02), clone 1B2
- Antibody type
- Monoclonal
- Description
- Mouse monoclonal antibody raised against a full length recombinant SPARC.
- Antigen sequence
MRAWIFFLLCLAGRALAAPQQEALPDETEVVEETV
AEVTEVSVGANPVQVEVGEFDDGAEETEEEVVAEN
PCQNHHCKHGKVCELDENNTPMCVCQDPTSCPAPI
GEFEKVCSNDNKTFDSSCHFFATKCTLEGTKKGHK
LHLDYIGPCKYIPPCLDSELTEFPLRMRDWLKNVL
VTLYERDEDNNLLTEKQKLRVKKIHENEKRLEAGD
HPVELLARDFEKNYNMYIFPVHWQFGQLDQHPIDG
YLSHTELAPLRAPLIPMEHCTTRFFETCDLDNDKY
IALDEWAGCFGIKQKDIDKDLVI- Isotype
- IgG
- Antibody clone number
- 1B2
- Storage
- Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
Submitted references The matricellular protein SPARC supports follicular dendritic cell networking toward Th17 responses.
Piconese S, Costanza M, Tripodo C, Sangaletti S, Musio S, Pittoni P, Poliani PL, Burocchi A, Passafaro AL, Gorzanelli A, Vitali C, Chiodoni C, Barnaba V, Pedotti R, Colombo MP
Journal of autoimmunity 2011 Dec;37(4):300-10
Journal of autoimmunity 2011 Dec;37(4):300-10
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- SPARC monoclonal antibody (M02), clone 1B2 Western Blot analysis of SPARC expression in A-431 ( Cat # L015V1 ).
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Western Blot analysis of SPARC expression in transfected 293T cell line by SPARC monoclonal antibody (M02), clone 1B2.Lane 1: SPARC transfected lysate(34.6 KDa).Lane 2: Non-transfected lysate.
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- SPARC monoclonal antibody (M02), clone 1B2. Western Blot analysis of SPARC expression in human kidney.
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Detection limit for recombinant GST tagged SPARC is approximately 3ng/ml as a capture antibody.
- Validation comment
- Sandwich ELISA (Recombinant protein)
- Protocol
- Protocol
