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Western blot
ELISAAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [1]
- Immunohistochemistry [1]
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Validation data
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- Product number
- AP05261PU-N - Provider product page
- Provider
- Acris Antibodies GmbH
- Proper citation
- Acris Antibodies GmbH Cat#AP05261PU-N, RRID:AB_1622573
- Product name
- anti SPARC / Osteonectin
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide derived from the Human SPARC protein
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Vial size
- 70 µg
- Concentration
- 0.29 mg/ml
Submitted references SPARC is a VCAM-1 counter-ligand that mediates leukocyte transmigration.
Kelly KA, Allport JR, Yu AM, Sinh S, Sage EH, Gerszten RE, Weissleder R
Journal of leukocyte biology 2007 Mar;81(3):748-56
Journal of leukocyte biology 2007 Mar;81(3):748-56
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Supportive validation
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Western Blot: Detection of endogenous SPARC in mouse heart lysate using AP05261PU-N (0.75 µg/ml) and anti-rabbit HRP (1:75k) and using Pierce's Super Signal West Femto.
Supportive validation
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Immunohistochemistry: Detection of endogenous SPARC in human hepatocellular carcinoma (HCC). 2 µg/ml AP05261PU-N, developed with Vector ImmPress anti-rabbit HRP and DAB substrate.
